HAS2 Antibody (C-5)recommended for detection of HAS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HAS2 Antibody (C-5): sc-365263

HAS2 Antibody (C-5) is rated 5.0 out of 5 by 4.
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Datasheets
    • HAS2 Antibody (C-5) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 489-517 within a C-terminal cytoplasmic domain of HAS2 of human origin
    • recommended for detection of HAS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-365263 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HAS2 (A-7): sc-514737 for HAS2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HAS2 (C-5): sc-365263.
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Can you recommend a negative control to use with HAS2 (C-5): sc-365263 monoclonal antibody?

Asked by: jenni
We recommend using the blocking peptide sc-365263 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 8
Date published: 2017-01-12

Can HAS2 (C-5): sc-365263 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, HAS2 (C-5): sc-365263 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-01-12
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Rated 5 out of 5 by from Outstanding !! Very specific western blot data; also great product for IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by from Good HAS2 antibody Good HAS2 detection in Western blot and staining. Works with mouse, rat, and human samples for WB, IP, IF, IHC(P), and ELISA.
Date published: 2017-01-27
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Nice Western Blot data of HAS2 expression Nice Western Blot data of HAS2 expression in mouse embryo tissue extract. -SCBT QC
Date published: 2013-04-25
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