HA-Tag Antibody (F-7): sc-7392

HA-Tag Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that targets the HA-Tag epitope, corresponding to amino acids 98-108 of the influenza hemagglutinin (HA) protein. HA (F-7) antibody recognizes a trimeric structure, with each monomer comprising two subunits, HA1 and HA2, linked by disulfide bonds, a configuration essential for mediating viral attachment to host cells and membrane fusion. This structural organization enables precise interactions with host cell receptors and facilitates conformational changes necessary for viral entry. The HA-Tag, derived from this well-characterized protein, retains structural specificity that makes an effective molecular marker for tagging and tracking proteins in various experimental systems. Anti-HA antibody (F-7) ensures strong and specific binding to accessible epitopes, enabling reliable use in immunohistochemistry with paraffin-embedded sections (IHCP), western blotting (WB), and immunoprecipitation (IP). Understanding the structure of hemagglutinin and its derived HA-Tag enhances the utility of this sequence in studying protein folding, interactions, and localization, which are fundamental to cellular and molecular biology. HA-Tag monoclonal antibody (F-7), available in conjugated and non-conjugated forms, supports diverse research applications by facilitating the detection and analysis of tagged proteins, helping researchers unravel complex structural and functional relationships in protein biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HA-Tag Antibody (F-7) References:

1. HAM: a new epitope-tag for in vivo protein labeling.  |  Herbst, A. and Tansey, WP. 2000. Mol Biol Rep. 27: 203-8. PMID: 11455955
2. Effects of N-linked glycosylation on the creatine transporter.  |  Straumann, N., et al. 2006. Biochem J. 393: 459-69. PMID: 16167890
3. Optimised cDNA size selection and cloning procedure for the construction of representative plasmid cDNA libraries.  |  Kieffer, BL. 1991. Gene. 109: 115-9. PMID: 1756970
4. The ocular albinism type 1 gene product, OA1, spans intracellular membranes 7 times.  |  Sone, M. and Orlow, SJ. 2007. Exp Eye Res. 85: 806-16. PMID: 17920058
5. Purification of epitope-tagged transcription factor IID.  |  Carey, MF., et al. 2010. Cold Spring Harb Protoc. 2010: pdb.prot5450. PMID: 20647354
6. Measuring protein-protein interactions using Biacore.  |  Leonard, P., et al. 2011. Methods Mol Biol. 681: 403-18. PMID: 20978978
7. Mapping HA-tagged protein at the surface of living cells by atomic force microscopy.  |  Formosa, C., et al. 2015. J Mol Recognit. 28: 1-9. PMID: 26046654
8. Measuring Protein-Protein Interactions Using Biacore.  |  Leonard, P., et al. 2017. Methods Mol Biol. 1485: 339-354. PMID: 27730561
9. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
10. A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection.  |  Kroll, DJ., et al. 1993. DNA Cell Biol. 12: 441-53. PMID: 7686011
11. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
12. A short polypeptide marker sequence useful for recombinant protein identification and purification.  |  Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlon, P.J. 1988. Nature Biotechnology. 6: 1204-1210.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HA-Tag Antibody (F-7) has 3,050 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HA-Tag Antibody (F-7) Specifications

Alternate Product Name: HA-Tag

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 98-108

Epitope Target: the influenza hemagglutinin (HA) protein

Epitope Species: influenza virus

Product Reactivity/Detection: proteins containing the HA tag

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3,050 citations in various scientific publications

Available Conjugates/Forms for HA-Tag Antibody (F-7): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent