granzyme B Antibody (2C5) recommended for detection of granzyme B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

granzyme B Antibody (2C5): sc-8022

granzyme B Antibody (2C5) is rated 4.9 out of 5 by 17.
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Datasheets
    • granzyme B Antibody (2C5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-247 representing full length granzyme B of human origin
    • recommended for detection of granzyme B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of granzyme B (2C5): sc-8022.
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Can I possibly know the sequence of amino acid peptide used for the production of this monoclonal antibody? Thank you very much!

Asked by: weiru
Thank you for your question. Yes, this information is available on our website and product data sheet. The antibody is not raised against a peptide, but rather a recombinant protein. granzyme B (2C5): sc-8022 is a mouse monoclonal raised against amino acids 1-247 representing full length granzyme B of human origin (corresponding to protein accession number: P10144).
Answered by: Technical Support
Date published: 2017-08-22

was it ever tested for flow cytometry application?

Asked by: weiru
Thank you for your question. We have not yet tested this antibody, granzyme B (2C5): sc-8022, for flow cytometry (FCM). Please contact our Technical Service Department if you have any additional questions. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-21

What is the light chain of granzyme B (2C5): sc-8022?

Asked by: cjMara
granzyme B (2C5) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 8
Date published: 2017-01-11

Which granzyme B clone do you recommend using?

Asked by: cjMara
We recommend using the granzyme B (2C5): sc-8022 over other clones available because it is validated in a variety of research applications, and cited in over a dozen publications.
Answered by: Technical Support 8
Date published: 2017-01-11
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Rated 3 out of 5 by from 效果不理想 用了1:500的稀释浓度 做人类来源等待肠道肿瘤组织和正常肠道组织 希望在免疫细胞胞质中有特异性染色 但肿瘤组织也有阳性表现 现在正在探索抗原修复时间和方式的改进 以及进一步稀释抗体 是否能有助于实验结果 感谢贵公司提供的试用产品
Date published: 2017-09-28
Rated 5 out of 5 by from Sensitive antibody Sensitive and specific for the detecting the granzyme B protein present in the positive cells. Successfully for western blot experiments that I use the dilution 1:500,great target band.
Date published: 2017-09-02
Rated 5 out of 5 by from tested for the specificity in WB It seems to be the only thoroughly tested granzyme B antibody for WB application! Refer to "Sedelies, K.A., Sayers, T.J., Edwards, K.M., Chen, W., Pellicci, D.G., Godfrey, D.I. and Trapani, J.A., 2004. Discordant regulation of granzyme H and granzyme B expression in human lymphocytes. Journal of Biological Chemistry, 279(25), pp.26581-26587."
Date published: 2017-08-20
Rated 5 out of 5 by from good experience Strong bands even at 1:1000 dilution for WB, I highly recommend
Date published: 2017-04-24
Rated 5 out of 5 by from Worked for human NK cell line This antibody worked great on western blot on human NK 92 cells. Single band!
Date published: 2017-02-17
Rated 5 out of 5 by from Good granzyme B antibody Good for granzyme B detection in mouse, rat, and human samples. Direct conjugates available for mouse on mouse staining, double or triple staining, or in general for bypassing secondary antibody. Strong signal obtained in multiple cell lines and tissue types.
Date published: 2017-01-23
Rated 5 out of 5 by from Best granzyme B antibody Superb western blot and IHC data; much better than polyclonal alternatives
Date published: 2017-01-15
Rated 5 out of 5 by from Works great We used this antibody and got great results on both mouse spleen and human melanoma.
Date published: 2016-12-07
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