Glycine A compound used for polyacrylamide gel electrophoresis and Western blots

Glycine (CAS 56-40-6)

Glycine | CAS 56-40-6 is rated 4.8 out of 5 by 6.
  • y_2019, m_1, d_16, h_3
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_29096, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 6ms
  • REVIEWS, PRODUCT
Synonym: Aminoacetic acid; Aminoethanoic acid; Glycocoll
Application: A compound used for polyacrylamide gel electrophoresis and Western blots
CAS Number: 56-40-6
Purity: ≥98%
Molecular Weight: 75.07
Molecular Formula: C2H5NO2
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers (Tris-Glycine; sc-296648) where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine (100 mM, pH 2.0-2.5; sc-295018) is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.


References

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Towbin, H., et al. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354. PMID: 388439
3. Shapiro, S., 1997. Neurosurgery. 40: 168-76; discussion 177. PMID: 8971839
4. Dubos, C., et al. 2003. Plant J. 35: 800-810. PMID: 12969432

Appearance :
Crystalline
Physical State :
Solid
Solubility :
Soluble in water (100 mg/ml), and acetone (slightly soluble). Insoluble in alcohol, and ether.
Storage :
Store at room temperature
Melting Point :
240° C
Boiling Point :
~240.90° C at 760 mmHg (Predicted)
Density :
1.60 g/cm3 at 20° C
Refractive Index :
n20D 1.46 (Predicted)
IC50 :
Glycine receptor alpha-4 chain: IC50 = 6 µM (mouse); Glutamate NMDA receptor: IC50 = 0.2 µM (rat)
Ki Data :
Glutamate NMDA receptor: Ki= 170 nM (rat)
pK Values :
pKa: 2.43 (Predicted), pKb: 9.64 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
MB7600000
PubChem CID :
750
Merck Index :
14: 4491
MDL Number :
MFCD00008131
EC Number :
200-272-2
Beilstein Registry :
635782
SMILES :
C(C(=O)O)N

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Glycine  Product Citations

See how others have used Glycine. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 19106099  Ray, S. et al. 2009. J. Biol. Chem. 284: 4978-4988.

PMID: # 17040914  Yevenes, GE. et al. 2006. J. Biol. Chem. 281: 39300-39307.

PMID: # 16790423  Marques, J.M. et al. 2006. J Biol Chem. 281: 24687-24694.

PMID: # 15037626  Henn, V. et al. 2004. J. Biol. Chem. 279: 26654-26665.

PMID: # 14600155  Rubio-Aliaga, I. et al. 2004. J Biol Chem. 279: 2754-2760.

PMID: # 15060063  Parathath, S. et al. 2004. J. Biol. Chem. 279: 24976-24985.

PMID: # 15319423  Yang, Li et al. 2004. J. Biol. Chem. 279: 46253-46262.

PMID: # 12414797  Ren, H. et al. 2003. J. Biol. Chem. 278: 276-283.

PMID: # 10455159  Zhang, S. et al. 1999. J. Biol. Chem. 274: 24865-24872.

PMID: # 10514479  Grossel, MJ. et al. 1999. J. Biol. Chem. 274: 29960-29967.

Citations 1 to 10 of 13 total

Good Morning. He wondered if it was possible to give a sample of glycine. We are currently working with another brand, but we are interested in testing your product. Thank you

Asked by: Ana Monteiro
Thank you for your question. We do not offer a sample of glycine. Please contact Technical Service if you have any further questions.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-15

Is this product ACS Certified?

Asked by: two2igm05
Thank you for your question. Glycine, sc-29096, is not ACS grade.
Answered by: Chemical Support 4
Date published: 2017-03-10
  • y_2019, m_1, d_16, h_3CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_29096, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 35ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great product We use it for our western blot. Works well. Strongly recommend it.
Date published: 2017-09-27
Rated 5 out of 5 by from Works great for western Works great for western buffer. Recommended to give a try.
Date published: 2017-08-03
Rated 5 out of 5 by from good package we purchased this bulk of glycine and works really well
Date published: 2017-05-17
Rated 5 out of 5 by from Great for our experiments The glycine ordered from Santa Cruz Biotech was much cheaper compared to other companies, but works great for our western blot.
Date published: 2017-01-17
Rated 4 out of 5 by from I will rate this product a 4 and rate 5 I will rate this product a 4 and rate 5 for your logistics, customer service and support.
Date published: 2015-04-20
Rated 5 out of 5 by from Why pay more for the same product somewhere else Why pay more for the same product somewhere else? Excellent value.
Date published: 2015-03-18
  • y_2019, m_1, d_16, h_3
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_29096, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 5ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.