Gas6 Antibody (A-9) recommended for detection of Gas6 of human origin by WB, IP, IF and ELISA

Gas6 Antibody (A-9): sc-376087

Gas6 Antibody (A-9) is rated 4.4 out of 5 by 8.
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Datasheets
    • Gas6 Antibody (A-9) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 481-620 mapping near the C-terminus of Gas6 of human origin
    • recommended for detection of Gas6 of human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gas6 (A-9): sc-376087.
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想问一下,这个小鼠源的抗体,能做大鼠或者小鼠组织的Western和免疫组化吗,谢谢!

Asked by: yang200214yy
Thank you for your question. Gas6 Antibody (A-9) is recommended for detection of Gas6 of human origin,We have not yet tested this antibody on mouse and rat origin.So the antibody is not recommended for mouse and rat sample at this time. 感谢您的提问。 Gas6 (A-9) 推荐检测人来源样本,未在大小鼠样本中做过相关验证,因此不推荐检测大小鼠实验样本。
Answered by: Technical Support 9
Date published: 2019-07-19

Is this Anti Gas6 Antibody ( (A-9): sc-376087) cross reactive with both human and mouse one?

Asked by: Nicki
Thank you for your question. Gas6 Antibody (A-9): sc-376087 is recommended for detection of Gas6 of human origin by WB, IP, IF and ELISA. It is not recommended for detection of Gas6 of murine origin, however, we cannot exclude cross-reactivity with murine samples. For further information, please contact our technical service directly.
Answered by: Tech Support Europe
Date published: 2019-05-08

Would this antibody be good for Neutralizing Gas6 from conditioned media from mouse cells?

Asked by: Nickhj89
Thank you for your question. This antibody has not been tested in any neutralizing research application.
Answered by: Technical Support
Date published: 2019-04-13

What is the recommended starting dilution for Western blot for sc-376087: Gas6 (A-9) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from This antibody works great for WB A single clear band between 50 - 75 kDa in immunoblot can be detected by this antibody.
Date published: 2018-04-12
Rated 4 out of 5 by from Worked well I ran my sample on a 10% gel with a 1:300 dilution and a 1:5000 secondary dilution. Came out clean after a 4th wash, nothing non-specific.
Date published: 2017-10-10
Rated 5 out of 5 by from Works great! Found this antibody to work great at a 1:1000. Will be using it from now on. Thanks!
Date published: 2017-08-24
Rated 5 out of 5 by from 之前听同事推荐使用贵公司抗体 我现在在研究炎症相关的蛋白以及生长因子,是经同学推荐使用贵公司的抗体,同学说贵公司的抗体效果不错,所以想先尝试一下,如果用的好就买,祝贵公司生意兴隆
Date published: 2017-06-23
Rated 5 out of 5 by from Solid antibody! Specific and reliable, this is a great choice for detecting Gas6, especially in Western blot.
Date published: 2017-02-02
Rated 1 out of 5 by from Cannot get bands in Western Blot Cannot get bands in Western Blot
Date published: 2015-03-25
Rated 5 out of 5 by from Strong band detected in transfected cell lysates Strong band detected in transfected cell lysates -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Positive titer in ELISA Positive titer in ELISA -SCBT QC
Date published: 2013-02-07
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