Fluorogenic Proteasome Substrate A fluorogenic substrate for measuring the peptidase activity of the 20S proteasome

Fluorogenic Proteasome Substrate (CAS 141223-71-4)

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Synonym: Cbz-Val-Lys-Met-Aminomethylcoumarin; Proteasome Substrate IV (fluorogenic); Z-VKM-AMC
Application: A fluorogenic substrate for measuring the peptidase activity of the 20S proteasome
CAS Number: 141223-71-4
Purity: ≥98% (HPLC)
Molecular Weight: 667.8
Molecular Formula: C34H45N5O7S
* Refer to Certificate of Analysis for lot specific data (including water content).
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Fluorogenic Proteasome Substrate (Z-VKM-AMC) is used to measure the peptidase activity of the 20S proteasome. Also used as a fluorogenic substrate for Alzheimer's Disease amyloid A4-generating enzymes.

AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.


References

1. Ishiura, S., et al., 1990. Distribution of Alzheimer's disease amyloid A4-generating enzymes in rat brain tissue. Neuroscience letters. 115(2-3): 329-34. PMID: 2234509
2. Koike, H., et al., 1999. Thimet oligopeptidase cleaves the full-length Alzheimer amyloid precursor protein at a beta-secretase cleavage site in COS cells. Journal of biochemistry. 126(1): 235-42. PMID: 10393344
3. Matsui, Hitoshi., et al., 2002. Presence of angiotensin-converting enzyme in follicular fluids of porcine ovaries and its possible involvement in the intrafollicular breakdown of bradykinin. Molecular reproduction and development. 62(1): 99-105. PMID: 11933166
4. Hook, Vivian Y H., et al., 2002. Beta-amyloid peptide in regulated secretory vesicles of chromaffin cells: evidence for multiple cysteine proteolytic activities in distinct pathways for beta-secretase activity in chromaffin vesicles. Journal of neurochemistry. 81(2): 237-56. PMID: 12064471
5. Hook, Vivian., et al., 2005. Inhibition of cathepsin B reduces beta-amyloid production in regulated secretory vesicles of neuronal chromaffin cells: evidence for cathepsin B as a candidate beta-secretase of Alzheimer's disease. Biological chemistry. 386(9): 931-40. PMID: 16164418
6. Hook, Vivian Y H., et al., 2008. Inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant, beta-secretase site of the amyloid precursor protein. The Journal of biological chemistry. 283(12): 7745-53. PMID: 18184658
7. Hook, Vivian., et al., 2012. Cysteine Cathepsins in the secretory vesicle produce active peptides: Cathepsin L generates peptide neurotransmitters and cathepsin B produces beta-amyloid of Alzheimer's disease. Biochimica et biophysica acta. 1824(1): 89-104. PMID: 21925292

Usage :
Following reconstitution, aliquot and freeze (-20°C); stable for up to 3 months at -20°C.
Appearance :
Lyophilized
Physical State :
Solid
Sequence :
Z-Val-Lys-Met-AMC
Solubility :
Soluble in DMSO.
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
3272309
SMILES :
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCSC)NC(=O)C(CCCCN)NC(=O)C(C(C)C)NC(=O)OCC3=CC=CC=C3

Download SDS (MSDS)

Certificate of Analysis

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