FLUO 3, Pentaammonium Salt A Ca2+ chelator and probe

FLUO 3, Pentaammonium Salt (CAS 134907-84-9)

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Application: A Ca2+ chelator and probe
CAS Number: 134907-84-9
Molecular Weight: 854.8
Molecular Formula: C36H30Cl2N2O13 5NH3
* Refer to Certificate of Analysis for lot specific data (including water content).
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FLUO 3, Pentaammonium Salt is a Ca2+ chelator and probe which operates at longer wavelengths of excitation. This offers less interference from endogenous autofluorescence, as well as the possibility of simultaneous use with other fluorescent probes.


References

New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures: R.Y. Tsien; Biochemistry 19, 2396 (1980) Fluorescence measurement and photochemical manipulation of cytosolic free calcium: R.Y. Tsien; TINS 11, 419 (1988) Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescence: M. Eberhard & P. Erne; BBRC 163, 309 (1989) Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores: A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) Photochemically generated cytosolic calcium pulses and their detection by fluo-3: J.P.Y. Kao, et al.; J. Biol. Chem. 264, 8179 (1989) Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+: J.E. Merritt, et al.; Biochem. J. 269, 513 (1990) Improved method for measuring intracellular Ca++ with fluo-3: G.T. Rijkers, et al.; Cytometry 11, 923 (1990) Control of mitochondrial matrix calcium: studies using fluo-3 as a fluorescent calcium indicator: A. Saavedra-Molina, et al.; BBRC 167, 148 (1990) Effects of photolabile calcium chelators on fluorescent calcium indicators: R.S. Zucker; Cell Calcium 13, 29 (1992)

Physical State :
Solid
Solubility :
Soluble in water, and alkaline solution.
Storage :
Store at -20° C
Fluoresence :
λex 506 nm, λem 526 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
44134721
MDL Number :
MFCD00133325
SMILES :
CC1=CC(=CC(=C1)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)O)Cl)N(CC(=O)[O-])CC(=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[NH4+].[NH4+].[NH4+].[NH4+]

Download SDS (MSDS)

Certificate of Analysis

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