ferritin heavy chain Antibody (B-12)recommended for detection of ferritin heavy chain of mouse, rat, human origin by WB, IP, IF and ELISA

ferritin heavy chain Antibody (B-12): sc-376594

ferritin heavy chain Antibody (B-12) is rated 4.8 out of 5 by 10.
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Datasheets
    • ferritin heavy chain Antibody (B-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 131-183 of ferritin heavy chain of human origin
    • recommended for detection of ferritin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ferritin heavy chain (B-12): sc-376594.
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Did you teste this antibody for detection of mouse Fth? Although it is recomended, I would like to know if it was actually tested.

Asked by: dlcosta83
Thank you for your question. Ferritin heavy chain Antibody (B-12): sc-376594 is recommended for detection of ferritin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA and has been established for those species and applications in our labs. We base our recommendations on these tests. The antibody has also been used successfully with murine samples in a recent publication: PMID 30935115.
Answered by: Tech Support Europe
Date published: 2019-05-27

I wonder how was this antibody validated to not cross react with Ferritin light chain?, has it been tried to be plotted with ferritin composed out of only light chain (like Ferritin from spleen)? I would like to use it in sandwich ELISA

Asked by: Zaher
Thank you for your question. This monoclonal antibody is raised against an antigen that is specific to the ferritin heavy chain, and is not expected to cross-react with ferritin light chain. We have not tested this antibody in spleen.
Answered by: Technical Support
Date published: 2018-12-10

Hello, I would like to know if this antibody can be used to detect rat ferritin heavy chain? Thank you.

Asked by: Teddy Chen
Thank you for your question. We have not yet tested our monoclonal antibody, ferritin heavy chain (B-12): sc-376594, to confirm reactivity with rat samples. However, it is very likely that the antibody would react with rat ferritin heavy chain based on roughly 92% sequence identity shared between the immunogen and the published protein sequence for rat (accession: P19132). We are offering a free 10 µg sample of this antibody for a limited time. Please contact our Technical Service Department if you would like a sample to test the antibody in rat. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-02

What is the recommended secondary to use in Western Blot with this antibody?

Asked by: cjMara
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, Mouse IgG kappa BP HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-19

Does this antibody (B12) cross-react with the ferritin light chain ?

Asked by: sv24
Thank you for your question. We do not expect this antibody, ferritin heavy chain Antibody (B-12): sc-376594, to cross-react with the ferritin light chain. The immunogen used to develop the antibody does not share significant sequence identity with the ferritin light chain protein. However, the exact epitope recognized by this monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-17
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Rated 5 out of 5 by from WB, 1:200, KM12 Working perfect for WB (1:200) in KM12 cell lysates (50 ug). No background, no unspecific bands.
Date published: 2017-10-11
Rated 4 out of 5 by from FTH The FTH Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date published: 2017-07-31
Rated 4 out of 5 by from 有两条带 这个抗体出现两个条带,我用的rat来源的细胞样品,一套在20kd左右,条带很浅,一条在35kd左右,条带深稀释倍数500。
Date published: 2017-07-17
Rated 5 out of 5 by from excellent for WB Strong, clear and right size band has been gotten by this specific antibody
Date published: 2017-04-11
Rated 5 out of 5 by from Great antibody for Western Blot The antibody recognize a single band corresponding to the heavy chain of ferritin. In some cases can also recognize ferritin light chain.
Date published: 2017-03-23
Rated 5 out of 5 by from Superb western Very clear western blot data; strong signal with low background
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of ferritin heavy chain expression in Caco-2, U-87 MG and HeLa whole cell lysates.
Date published: 2017-01-17
Rated 5 out of 5 by from The result of Western bloting is very good! Antibody dilution ratio is moderate, better exposure, the map of article perfect
Date published: 2016-11-15
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