ferritin heavy chain Antibody (B-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against amino acids 131-183 of ferritin heavy chain of human origin
recommended for detection of ferritin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ferritin heavy chain (B-12): sc-376594.
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Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Did you teste this antibody for detection of mouse Fth? Although it is recomended, I would like to know if it was actually tested.
Asked by: dlcosta83
Thank you for your question. Ferritin heavy chain Antibody (B-12): sc-376594 is recommended for detection of ferritin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA and has been established for those species and applications in our labs. We base our recommendations on these tests. The antibody has also been used successfully with murine samples in a recent publication: PMID 30935115.
Answered by: Tech Support Europe
Date published: 2019-05-27
I wonder how was this antibody validated to not cross react with Ferritin light chain?, has it been tried to be plotted with ferritin composed out of only light chain (like Ferritin from spleen)?
I would like to use it in sandwich ELISA
Asked by: Zaher
Thank you for your question. This monoclonal antibody is raised against an antigen that is specific to the ferritin heavy chain, and is not expected to cross-react with ferritin light chain. We have not tested this antibody in spleen.
Answered by: Technical Support
Date published: 2018-12-10
I would like to know if this antibody can be used to detect rat ferritin heavy chain? Thank you.
Asked by: Teddy Chen
Thank you for your question. We have not yet tested our monoclonal antibody, ferritin heavy chain (B-12): sc-376594, to confirm reactivity with rat samples. However, it is very likely that the antibody would react with rat ferritin heavy chain based on roughly 92% sequence identity shared between the immunogen and the published protein sequence for rat (accession: P19132).
We are offering a free 10 µg sample of this antibody for a limited time. Please contact our Technical Service Department if you would like a sample to test the antibody in rat. Please call our European Support office toll-free at 00800 4573 8000, email firstname.lastname@example.org, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-02
What is the recommended secondary to use in Western Blot with this antibody?
Asked by: cjMara
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, Mouse IgG kappa BP HRP, sc-516102. A complete list of available binding proteins is available on our website here:
Answered by: Technical Service 11
Date published: 2017-01-19
Does this antibody (B12) cross-react with the ferritin light chain ?
Asked by: sv24
Thank you for your question. We do not expect this antibody, ferritin heavy chain Antibody (B-12): sc-376594, to cross-react with the ferritin light chain. The immunogen used to develop the antibody does not share significant sequence identity with the ferritin light chain protein. However, the exact epitope recognized by this monoclonal antibody has not been determined.
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