F4/80 Antibody (C-7) recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA

F4/80 Antibody (C-7): sc-377009

F4/80 Antibody (C-7) is rated 4.4 out of 5 by 20.
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Datasheets
    • F4/80 Antibody (C-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 335-634 mapping within an extracellular domain of F4/80 of mouse origin
    • recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of F4/80 (C-7): sc-377009.
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Can this antibody be used in paraffin-embedded sections?

Asked by: Anonymous
Thank you for your question. Yes, F4/80 (C-7): sc-377009 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-09

What is a good positive control to use with the SIRT1 (B-7) sc-74465 antibody?

Asked by: Anonymous
We recommend K-562 nuclear extract: sc-2130, RAW 264.7 whole cell lysate: sc-2211, HL-60 whole cell lysate: sc-2209, M1 whole cell lysate: sc-364782, and WEHI-3 whole cell lysate: sc-3815. We have Western blot data showing specific detection of SIRT1 in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-09

Can this antibody be used in FCM to screen the macrophage of rat?

Asked by: Linlic
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Rated 4 out of 5 by from Good in IHC Used on human cancer cells, and have good signals when using it.
Date published: 2017-10-19
Rated 5 out of 5 by from perfect in IF I used this antibody before 4 days ago. The product shows perfect quality. The samples are breast cancer masses of BalB/C mice. Macrophages were detected clearly with F4/80 antibody. The dilution was 1:200.
Date published: 2017-09-14
Rated 3 out of 5 by from F4/80 is no bind WB 1:1000 5%BSA Block. primary antibody 4℃ overnight secondary antibody HRP, RT, 1hour.
Date published: 2017-09-02
Rated 4 out of 5 by from Great Macrophages in rat lung tissue were detected with F4/80 antibody, and F4/80 antibody worked well on IF of paraffin sections. We started dillution was 1:200.
Date published: 2017-08-15
Rated 1 out of 5 by from Not working I checked this Ab for mouse tissue staining, but got no signal.
Date published: 2017-08-14
Rated 5 out of 5 by from Wonderful Result! I used it for IF (frozen section) of mouse tumor at a dilution of 1:200 to identify macrophages in tumor tissue, and the staining result was wonderful!
Date published: 2017-08-13
Rated 5 out of 5 by from happy result I am happy with the result,I recommend use dilution 1:400 for best dilution,specific band obatined.
Date published: 2017-06-14
Rated 5 out of 5 by from GREAT!!! It works well in IHC at a dilution 1:100,I appreciate it very much.
Date published: 2017-05-25
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