Electrophoresis Sample Buffer, 2X A ready-to-use reducing sample buffer for SDS-PAGE

Electrophoresis Sample Buffer, 2X

Electrophoresis Sample Buffer, 2X is rated 4.3 out of 5 by 7.
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Application: A ready-to-use reducing sample buffer for SDS-PAGE
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included as a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer contains DTT and TCEP-HCl, which disrupt disulfide bonds to ensure proteins are fully denatured before loading on the gel.
Contains: glycerol (sc-29095), TCEP-HCl (sc-203290), DTT (sc-29089), SDS (sc-24950), MES (sc-255262), EDTA (sc-286969), Triton X-100 (sc-29112) and bromophenol blue (sc-24971).
For non-reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (non-reducing) (sc-45085) is recommended.


References

1. Toyoda, E., et al. 2005. Pancreas. 30: 168-173. PMID: 15714139
2. Heinz, L.X., et al. 2006. Blood. 107: 1445-1453. PMID: 16223775
3. Gohil, K., et al. 2008. Brain Res. 1201: 167-176. PMID: 18299118
4. Popovici, R.M., et al. 2008. Endocrinology. 149: 1136-1143. PMID: 18048494
5. Tang, Y., et al. 2010. J. Nutr. Biochem. 21: 237-246. PMID: 19369054

Usage :
Mix sample with an equal volume of Electrophoresis Sample Buffer, 2X. Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
Appearance :
Aqueous solution
Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Electrophoresis Sample Buffer, 2X  Product Citations

See how others have used Electrophoresis Sample Buffer, 2X. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 29 total

PMID: # 26514550  Winiarska, K. et al. 2016. Journal of pineal research. 60: 109-17.

PMID: # 26734660  Peng, S. et al. 2015. Annals of clinical and translational neurology. 2: 1085-104.

PMID: # 26432891  Talaei, N. et al. 2015. J. Immunol. 195: 4623-31.

PMID: # 24370615  Joiner, DM. et al. 2014. Calcified tissue international. 94: 484-94.

PMID: # 23291594  Wang, R. et al. 2013. Free radical biology & medicine. 57: 141-53.

PMID: # 23686888  Jia, Y. et al. 2013. Andrology. 1: 651-9.

PMID: # 23787122  Kaufman, JL. et al. 2013. Clinical lymphoma, myeloma & leukemia. 13: 370-6.

PMID: # 22297640  Miyagaki, T. et al. 2012. The Journal of investigative dermatology. 132: 1280-9.

PMID: # 21984910  Kozubowski, L. et al. 2011. PloS one. 6: e25280.

PMID: # 21599470  Das, S. et al. 2011. Curr. Eye Res. 36: 620-631.

Citations 1 to 10 of 29 total

沈殿物があり、転倒混和しても溶解しません。また浮遊している粒子がピペットで吸うときに詰まって、正確な量が吸えません。 粒子は沈殿させて上清を吸えば、製品として問題ないのでしょうか?ご教示ください。

Asked by: naganuma1031
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at asia@scbio.cn, or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メールasia@scbio.cnまたはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2017-10-27
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Rated 5 out of 5 by from Convenient to use Very convenient since DTT is already included in the solution. Saves time in western blot preparation
Date published: 2019-02-04
Rated 5 out of 5 by from The best one Really good one. I would definitely go for it again.
Date published: 2018-09-24
Rated 2 out of 5 by from Disappointed I am unhappy with this product because of 2 reasons: The products gets precipitated and difficult to pipette . The dye concentration is too low making it difficult to track the protein sample.
Date published: 2018-07-04
Rated 5 out of 5 by from Electrophoresis sample buffer Great sample buffer for protein gel electrophoresis, provided nice results.
Date published: 2018-03-28
Rated 5 out of 5 by from Very clear we use it detect the α E-catenin in cancer cell, we usually use the dilution 1:800, bands came out very clear.
Date published: 2017-04-29
Rated 5 out of 5 by from Results were great. Electrophoresis Buffer antibody provides clear, specific results and good specificity. On the whole, we are satisfied with the antibody.
Date published: 2017-02-07
Rated 3 out of 5 by from Precipitation occurs Precipitation occurs
Date published: 2015-04-03
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