EGFR Antibody (A-10) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EGFR Antibody (A-10): sc-373746

EGFR Antibody (A-10) is rated 4.7 out of 5 by 44.
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Datasheets
    • EGFR Antibody (A-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin
    • recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-373746 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of EGFR (A-10): sc-373746.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
EGFR Antibody (A-10) sc-373746 200 µg/ml $279.00
EGFR Antibody (A-10) AC sc-373746 AC 500 µg/ml, 25% agarose $366.00
EGFR Antibody (A-10) HRP sc-373746 HRP 200 µg/ml $279.00
EGFR Antibody (A-10) FITC sc-373746 FITC 200 µg/ml $292.00
EGFR Antibody (A-10) PE sc-373746 PE 200 µg/ml $303.00
EGFR Antibody (A-10) Alexa Fluor® 488 sc-373746 AF488 200 µg/ml $314.00
EGFR Antibody (A-10) Alexa Fluor® 546 sc-373746 AF546 200 µg/ml $314.00
EGFR Antibody (A-10) Alexa Fluor® 594 sc-373746 AF594 200 µg/ml $314.00
EGFR Antibody (A-10) Alexa Fluor® 647 sc-373746 AF647 200 µg/ml $314.00
EGFR Antibody (A-10) Alexa Fluor® 680 sc-373746 AF680 200 µg/ml $314.00
EGFR Antibody (A-10) Alexa Fluor® 790 sc-373746 AF790 200 µg/ml $314.00
EGFR (A-10) Blocking Peptide sc-373746 P 100 µg/0.5 ml $61.00

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EGFR Antibody (A-10)Product Citations

See how others have used EGFR Antibody (A-10). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 35 total

PMID: # 29321665  Sun, L. et al. 2018. Oncogene. 37: 1386-1398.

PMID: # 29607865  Cevik, S. et al. 2018. Niger J Clin Pract. 21: 502-506.

PMID: # 29038347  Suyama, K. et al. 2018. Cancer research. 78: 103-114.

PMID: # 30108171  Watkins, TN. et al. 2018. MBio. 9:

PMID: # 30009856  Yoon, YJ. et al. 2018. Cancer Lett. 434: 42-55.

PMID: # 29768059  Shih-Wei, W. et al. 2018. J Enzyme Inhib Med Chem. 33: 920-935.

PMID: # 30115673  Karki, K. et al. 2018. Mol. Pharmacol. 94: 1187-1196.

PMID: # 30021363  Lu, T. et al. 2018. Biomed. Pharmacother. 105: 1269-1275.

PMID: # 30249613  Rush, JS. et al. 2018. Mol. Pharmacol.

PMID: # 28561591  ElHady, AK. et al. 2017. J. Med. Chem. 60: 5377-5391.

Citations 1 to 10 of 35 total

We recently ran the EGFR Antibody (A-10): sc-373746 and did not detect any banding on U87 lysates. What positive control do you recommend. We rant the lysate on a 4-12% MOPS PAGE....

Asked by: DrxFif
Thank you for your inquiry. We recommend serum starved Hela or A549 cells, or HepG2 cells. 4-12% gel should be fine. Please contact our Technical Service Department by phone, e-mail or live chat, and we'd be happy to help you troubleshoot the antibody.
Answered by: Tech Service
Date published: 2017-08-14

What is the recommended antigen retrieval method to be used with this antibody?

Asked by: mony8
Thank you for your question. We recommend using a sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this mouse monoclonal antibody, EGFR (A-10): sc-373746. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-06-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23

if I probe western blot with EGFR sc-373746, I don't have to against the blot with 2nd antibody. Is that right? Is this a phospho-EGFR antibody?

Asked by: Daisy II

HI, I need to do negative depletion of cells using an anti-EGF-receptor monoclonal antibody, mouse-human. can I use EGFR Antibody (A-10): sc-373746 for this purpose?

Asked by: Maria Vernon
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Rated 4 out of 5 by from WB HEK293T細胞でEGF-EGFRアッセイを行い、きれいなバンドが予想通りの高さに確認されました。
Date published: 2018-09-22
Rated 5 out of 5 by from Good antibody for WB I received this antibody as a free monoclonal trial and I will buy it again for Western blot application.
Date published: 2018-09-10
Rated 4 out of 5 by from Worked well for WB analysis Tried 1:500 with human bladder cancer cells for WB analysis - strong signal detected
Date published: 2018-06-21
Rated 5 out of 5 by from WB iPS細胞から分化したcortical neuronのEGFRをWBで認識し、きれいなバンドが検出されました。
Date published: 2018-04-19
Rated 3 out of 5 by from Good I tested in HUH7 cells, and it did work, but it did not work better as the previous one.
Date published: 2018-03-30
Rated 5 out of 5 by from Great results for WB Great results for WB when probing in vivo mouse samples at 1:200. Highly recommend!
Date published: 2018-03-22
Rated 5 out of 5 by from Absolutely beautiful for western blot Probably one of the best antibodies I have ever used. I used 100 ug of protein from tissue at 1:1000 dilution. This was probably even overkill. Crystal clean and clear blot no background.
Date published: 2018-02-21
Rated 4 out of 5 by from Good Ab It worked and signal was strong but need to use in higher concentration.
Date published: 2018-02-14
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