EGFR Antibody (A-10) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EGFR Antibody (A-10): sc-373746

EGFR Antibody (A-10) is rated 4.7 out of 5 by 47.
  • y_2019, m_6, d_14, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_46
  • loc_en_US, sid_373746, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 93ms
  • REVIEWS, PRODUCT
Datasheets
    • EGFR Antibody (A-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin
    • recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-373746 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EGFR (A-10): sc-373746.
Submit a review for this product and receive 15 CruzCredits

HI, I need to do negative depletion of cells using an anti-EGF-receptor monoclonal antibody, mouse-human. can I use EGFR Antibody (A-10): sc-373746 for this purpose?

Asked by: Maria Vernon
Thank you for your question. We have not tested this antibody for any biological activity such as depletion, and so this is not a recommended application at this time. We do offer an alternative antibody which has been validated for use in biological studies, including neutralization, EGFR Antibody (R-1): sc-101, also provided azide-free for biological studies, sc-101 L.
Answered by: Technical Support
Date published: 2019-03-03

Is it positive for MCF-7 or MDA-MB-231 ?

Asked by: Gracess
Thank you for your question. We have published Western blot data on our website using this antibody in MDA-MB-231 whole cell lysates. We do not have any data in MCF-7.
Answered by: Technical Support
Date published: 2019-01-31

We recently ran the EGFR Antibody (A-10): sc-373746 and did not detect any banding on U87 lysates. What positive control do you recommend. We rant the lysate on a 4-12% MOPS PAGE....

Asked by: DrxFif
Thank you for your inquiry. We recommend serum starved Hela or A549 cells, or HepG2 cells. 4-12% gel should be fine. Please contact our Technical Service Department by phone, e-mail or live chat, and we'd be happy to help you troubleshoot the antibody.
Answered by: Tech Service
Date published: 2017-08-14

What is the recommended antigen retrieval method to be used with this antibody?

Asked by: mony8
Thank you for your question. We recommend using a sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this mouse monoclonal antibody, EGFR (A-10): sc-373746. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-06-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23

if I probe western blot with EGFR sc-373746, I don't have to against the blot with 2nd antibody. Is that right? Is this a phospho-EGFR antibody?

Asked by: Daisy II
  • y_2019, m_6, d_14, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_en_US, sid_373746, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 83ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very good for WB Worked very nice in Western Blot with human samples.
Date published: 2019-04-18
Rated 5 out of 5 by from Fabulous features This antibody worked great for western blot and images are very good.
Date published: 2019-03-29
Rated 5 out of 5 by from GOOD antibody This antibody was diluted in 1:500,and the results is good!
Date published: 2018-12-24
Rated 4 out of 5 by from WB HEK293T細胞でEGF-EGFRアッセイを行い、きれいなバンドが予想通りの高さに確認されました。
Date published: 2018-09-22
Rated 5 out of 5 by from Good antibody for WB I received this antibody as a free monoclonal trial and I will buy it again for Western blot application.
Date published: 2018-09-10
Rated 4 out of 5 by from Worked well for WB analysis Tried 1:500 with human bladder cancer cells for WB analysis - strong signal detected
Date published: 2018-06-21
Rated 5 out of 5 by from WB iPS細胞から分化したcortical neuronのEGFRをWBで認識し、きれいなバンドが検出されました。
Date published: 2018-04-19
Rated 3 out of 5 by from Good I tested in HUH7 cells, and it did work, but it did not work better as the previous one.
Date published: 2018-03-30
  • y_2019, m_6, d_14, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_46
  • loc_en_US, sid_373746, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 27ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.