DTT

DTT (CAS 27565-41-9)

DTT | CAS 27565-41-9 is rated 4.7 out of 5 by 3.
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Synonym: DL-Dithiothreitol; Cleland's Reagent; Dithiothreitol
Application: A cell permeable protective agent for Thiol groups
CAS Number: 27565-41-9
Purity: >97%
Molecular Weight: 154.25
Molecular Formula: C4H10O2S2
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

DTT (1,4-Dithiothreitol) acts as a cell permeable protective agent for Thiol groups. Keeps monothiols completely in the reduced state and reduces disulfides quantitatively. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. Since DTT readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation. Can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. It has been reported that a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol.


References

1. Cleland, W.W., et al. 1964. Biochemistry. 3: 480-482. PMID: 14192894
2. Klonne, D.R., et al. 1988. Toxicol. Lett. 42: 199-205. PMID: 2841777
3. Lauriault, V.V., et al. 1991. Mol. Pharmacol. 40: 125-134. PMID: 1649964
4. Gailis, L. 1994. Eur. J. Pharmacol. 292: 39-42. PMID: 7867688

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (15.4 g/l) at 20 °C, ethanol, acetone, ethyl acetate, chloroform, and ether.
Storage :
Store at 4° C
Melting Point :
41-44° C
Boiling Point :
~364.5° C at 760 mmHg (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.58 (Predicted)
pK Values :
pKa: 9.28 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
EK1610000
Transport :
UN 3335, Class 9, Packing group III
PubChem CID :
19001
Merck Index :
14: 3376
MDL Number :
MFCD00004877
EC Number :
222-468-7
Beilstein Registry :
1719757
SMILES :
C(C(C(CS)O)O)S

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Certificate of Analysis

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DTT  Product Citations

See how others have used DTT. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 20 total

PMID: # 27053106  Popa, IL. et al. 2016. J. Biol. Chem.

PMID: # 26199612  Bae, EJ. et al. 2015. Neural Regen Res. 10: 944-50.

PMID: # 26141394  Wan, D. et al. 2015. Toxicol. Sci. 147: 326-38.

PMID: # 25351722  Ahn, JH. et al. 2015. Mol Med Rep. 11: 1043-8.

PMID: # 24962405  Ahn, JH. et al. 2014. J. Vet. Sci. 15: 335-42.

PMID: # 25551446  Khan Mirzaei, M. et al. 2014. PLoS ONE. 9: e116294.

PMID: # 24962405  Ahn, JH. et al. 2014. Journal of veterinary science. 15: 335-42.

PMID: # 12909330  Gündüz, D. et al. 2003. Cardiovasc. Res. 59: 470-8.

PMID: # 12486103  Stehlik, C. et al. 2002. J. Exp. Med. 196: 1605-1615.

PMID: # 11158315  Koumenis, C. et al. 2001. Mol. Cell. Biol. 21: 1297-1310.

Citations 1 to 10 of 20 total
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Rated 5 out of 5 by from DTT Review Used for western blot sample buffer, working well, happy with this product
Date published: 2018-08-10
Rated 5 out of 5 by from First time using this product and it worked First time using this product and it worked really well.
Date published: 2015-04-24
Rated 4 out of 5 by from I will rate this product a 4 and rate 5 I will rate this product a 4 and rate 5 for your logistics, customer service and support.
Date published: 2015-04-20
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