Dimethyl Sulfoxide (DMSO), cell culture reagent is ideal for cryopreservation. DMSO is a polar, aprotic organic solvent with many applications in chemical and biological research. DMSO can uniquely provide solubility to certain very polar small molecules, such as simple biomolecules with multiple protic functional groups. It can also be used as an oxidation reagent in certain transformations. The yield of PCR processes is amplified with DMSO as a co-solvent, helping to inhibit the formation of secondary structures from DNA fragments.
1. Sweat, F.W. and Epstein, W.W. 1967. J. Org. Chem. 32: 835-838. PMID: 6042131 2. Winship, P.R. 1989. Nucleic Acids Res. 17: 1266. PMID: 2922271 3. Chakrabarti, R. and Schutt, C.E. 2001. Gene. 274: 293-298. PMID: 11675022 4. Heidemann, R., et al. 2010. Biotechnol. Prog. 26: 1154-1163. PMID: 20730770 5. Thirumala, S., et al. 2010. Stem Cells Dev. 19: 513-522. PMID: 19788372
Dimethyl Sulfoxide (DMSO), Cell Culture reagent is used in molecular biology, especially in the polymerase chain reaction (PCR), cell lysis, in transformation and transfection and in cytofluorimetric assessment. DMSO is most often used at a concentration of 10%.
Soluble in water, methanol, acetone, ether, chloroform, benzene, and ethanol. DMSO is miscible in most of the common organic solvents such as alcohols, esters, ketones, ethers, chlorinated solvents and aromatics.
Desiccate at room temperature
16-19° C (lit.)
189° C at 101.3 kPa
n20D 1.48 (lit.)
HII4E cells : IC50 = 199 µg.mL-1 (mouse)
Choline acetylase: Ki= 25 mM (rat)
pKa: -4.54 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Download SDS (MSDS)
Certificate of Analysis
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Cuál es el formato del envase? cristal o plástico?
Asked by: RVirievac
Thank you for your question. This biochemical is provided as a liquid in a glass vial.
Answered by: Tech Service
Date published: 2019-04-09
I need your offer for 3x100ml?
1And information about transport costs to Serbia.
Mrs. Dragica Grudic
Asked by: Dragica grudic
The lead time and the shipping costs depend on the destination.
For more information please contact costumer care by phone or by live chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21
We want to order DMSO (undiluted-100%) for cell culture. I want to know if it has been tested for low endo-toxins.
Asked by: Harleen
Thank you for your question. Yes, endotoxin levels are tested for this cell culture grade DMSO. The specifications are provided on our CoA. Each lot of this chemical comes with a lot-specific Certificate Of Analysis. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to request a copy of the CoA for our most recent lot.
Answered by: Technical Support
Date published: 2018-06-05
Is this product sterilized?
Asked by: zhangmeng
Thank you for your question. This product is not sterilely filtered. It is cell culture grade and due to the toxicity of the solvent, not much will grow in it. You can sterile filter the item in the pure form using a nitrocellulose filter or using a regular filter when the DMSO is diluted. We do offer DMSO sterile filtered (see sc-359032). If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <email@example.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-09-14
What are the endotoxin levels in this chemical?
Asked by: Jz28sail
Thank you for your question. Endotoxin levels for sc-358801, Dimethyl Sulfoxide (DMSO), cell culture reagent are expected to be <2.5 EU/mL.
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