Diethanolamine (DEA) is a polyfunctional product that is a secondary amine and a diol and acts as a weak base. Diethanolamine buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5) is frequently used in colorimetric assays (ELISA) using p-nitrophenyl phosphate (PNPP, sc-3720) substrate and diethanolamine buffer. DEA (5% diethanolamine, pH 11.5) may also be useful for the dissociation of antigen-antibody complexes. For surface staining of monoclonal antibody-alkaline phosphatase conjugates by flow cytometric analysis, diethanolamine buffer (pH 9.6) has been used with BCIP/NBT (sc-24981) substrate. Also used as an absorbent for acid gases and as a chemical intermediate. Also available: PNPP substrate buffer: sc-296099.
1. Chong, P. and Gillam, S. 1985. J. Virol. Methods. 10: 261-268. PMID: 3988877 2. Kim, Y.R., et al. 1992. Am. J. Clin. Pathol. 97: 331-337. PMID: 1371900 3. Turan, S., et al. 2011. J Clin Res Pediatr Endocrinol. 3: 7-11. PMID: 21448327
Soluble in water (105 g/l), alcohol, methanol, and acetone. Insoluble in ether, and benzene.
Store at room temperature
28° C (lit.)
217° C (lit.) at 150 mmHg
1.10 g/mL at 25° C (lit.)
n20D 1.48 (lit.)
pKa: 8.88 in water at 25°C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Various publications cite the use of DiethanolamineVarious publications cite the use of Diethanolamine (DEA) in colorimetric assays (ELISA) using p-nitrophenyl phosphate (PNPP, sc-3720) substrate and diethanolamine buffer. For surface staining of monoclonal antibody-alkaline phosphatase conjugates by flow cytometric analysis, diethanolamine buffer (pH 9.6) has been used with BCIP/NBT (sc-24981) substrate. -SCBT Publication Review
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