DiethanolamineDEA, frequently used as a diluent for PNPP in ELISA assays

Diethanolamine (CAS 111-42-2)

Diethanolamine | CAS 111-42-2 is rated 5.0 out of 5 by 1.
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Synonym: 2,2'-Iminodiethanol
Application: DEA, frequently used as a diluent for PNPP in ELISA assays
CAS Number: 111-42-2
Molecular Weight: 105.14
Molecular Formula: C4H11NO2
* Refer to Certificate of Analysis for lot specific data (including water content).
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Diethanolamine (DEA) is a polyfunctional product that is a secondary amine and a diol and acts as a weak base. Diethanolamine buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5) is frequently used in colorimetric assays (ELISA) using p-nitrophenyl phosphate (PNPP, sc-3720) substrate and diethanolamine buffer. DEA (5% diethanolamine, pH 11.5) may also be useful for the dissociation of antigen-antibody complexes. For surface staining of monoclonal antibody-alkaline phosphatase conjugates by flow cytometric analysis, diethanolamine buffer (pH 9.6) has been used with BCIP/NBT (sc-24981) substrate. Also used as an absorbent for acid gases and as a chemical intermediate.
Also available: PNPP substrate buffer: sc-296099.


References

1. Chong, P. and Gillam, S. 1985. J. Virol. Methods. 10: 261-268. PMID: 3988877
2. Kim, Y.R., et al. 1992. Am. J. Clin. Pathol. 97: 331-337. PMID: 1371900
3. Turan, S., et al. 2011. J Clin Res Pediatr Endocrinol. 3: 7-11. PMID: 21448327

Physical State :
Low-Melting Solid
Solubility :
Soluble in water (105 g/l), alcohol, methanol, and acetone. Insoluble in ether, and benzene.
Storage :
Store at room temperature
Melting Point :
28° C (lit.)
Boiling Point :
217° C (lit.) at 150 mmHg
Density :
1.10 g/mL at 25° C (lit.)
Refractive Index :
n20D 1.48 (lit.)
pK Values :
pKa: 8.88 in water at 25°C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
KL2975000
PubChem CID :
8113
Merck Index :
14: 3107
MDL Number :
MFCD00002843
EC Number :
203-868-0
Beilstein Registry :
605315
SMILES :
C(CO)NCCO

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Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite the use of Diethanolamine Various publications cite the use of Diethanolamine (DEA) in colorimetric assays (ELISA) using p-nitrophenyl phosphate (PNPP, sc-3720) substrate and diethanolamine buffer. For surface staining of monoclonal antibody-alkaline phosphatase conjugates by flow cytometric analysis, diethanolamine buffer (pH 9.6) has been used with BCIP/NBT (sc-24981) substrate. -SCBT Publication Review
Date published: 2015-02-12
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