DBP Antibody (A-5) recommended for detection of DBP of human origin by WB, IP, IF, IHC(P) and ELISA

DBP Antibody (A-5): sc-365441

DBP Antibody (A-5) is rated 5.0 out of 5 by 3.
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Datasheets
    • DBP Antibody (A-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 175-474 mapping at the C-terminus of DBP of human origin
    • recommended for detection of DBP of human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DBP (A-5): sc-365441.
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I am using DBP (A-5): sc-365441 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works great on western blot We have used this antibody on human skin tissue. It works great on western blot.
Date published: 2017-09-20
Rated 5 out of 5 by from Produced superb immunoperoxidase membrane Produced superb immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-05-10
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