DAR-4M is a rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. DAR-4M's fluorescence intensity is not dependent on pH, unlike DAF-2.
1. Kojima, H., et al., 2001. Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. Analytical chemistry. 73(9): 1967-73. PMID: 11354477
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