DAB, 50X A product used in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins

DAB, 50X  (CAS 7411-49-6)

DAB, 50X | CAS 7411-49-6 is rated 5.0 out of 5 by 1.
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| See product citations (61)
Synonym: 3,3' Diaminobenzidine Tetrahydrochloride
Application: A product used in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins
CAS Number: 7411-49-6
Molecular Weight: 360.11
Molecular Formula: C12H14N44HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
DAB, 50X sc-24982 10 ml $75.00
DAB, 50X sc-24982A 100 ml $500.00

DAB (3-3′ diaminobenzidine tetrachloride) is suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins. In HRP-based assays, the buffer oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium.


References

1. Krueger, S.K., et al. 1999. Biotech. Histochem. 74: 105-109. PMID: 10333408
2. Gallyas, F., et al. 1982. J. Histochem. Cytochem. 30: 183-184. PMID: 7061820
3. Larrson, L.I. 1988. Immunocytochemistry: Theory and Practice. Boca Raton, FL. CRC Press, Inc.

Usage :
Dilute to 1x with 100mM Tris-HCl pH 7.6
Recommended for use with UltraCruz™ Mounting Medium (sc-24941).

Discard if there is precipitate in the solution prior to mixing with oxidizing agent. Discard if reagent is still purple after making solution with oxidizing agent.
Formulation :
50X concentrate
Appearance :
Solution
Physical State :
Liquid
Solubility :
Soluble in water (Dilutable). Insoluble in most organic solvents.
Storage :
Store at 4° C
Refractive Index :
n20D 1.77 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
23892
MDL Number :
MFCD00012969
EC Number :
231-018-9
SMILES :
C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.Cl.Cl.Cl.Cl

Download SDS (MSDS)

Certificate of Analysis

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DAB, 50X Product Citations

See how others have used DAB, 50X. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 61 total

PMID: # 26482544
Xing, R. et al. 2016. Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis. Scand. J. Immunol.. 83: 64-71.

PMID: # 27008403
Fan, M. et al. 2016. Rac1/β-Catenin Signalling Pathway Contributes to Trophoblast Cell Invasion by Targeting Snail and MMP9. Cell. Physiol. Biochem.. 38: 1319-32.

PMID: # 27208794
Mukherjee, N. et al. 2016. Frequent inactivation of MCC/CTNNBIP1 and overexpression of phospho-beta-catenin(Y654) are associated with breast carcinoma: Clinical and prognostic significance. Biochim. Biophys. Acta. 1862: 1472-84.

PMID: # 25465073
Maiti, GP. et al. 2015. Frequent inactivation of SLIT2 and ROBO1 signaling in head and neck lesions: clinical and prognostic implications. Oral Surg Oral Med Oral Pathol Oral Radiol. 119: 202-12.

PMID: # 26500110
Zhou, R. et al. 2015. Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars. Experimental cell research. 339: 360-6.

PMID: # 26500110
Zhou, R. et al. 2015. Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars. Exp. Cell Res.. 339: 360-6.

PMID: # 25543042
Wu, J. et al. 2014. The correlation between IL-20 and the Th2 immune response in human asthma. Asian Pac. J. Allergy Immunol.. 32: 316-20.

PMID: # 26339260
Jafari, S. et al. 2014. Recombinant production of mecasermin in E. coli expression system. Research in pharmaceutical sciences. 9: 453-61.

PMID: # 24656731
Zhang, L. et al. 2014. Regeneration of human-ear-shaped cartilage by co-culturing human microtia chondrocytes with BMSCs. Biomaterials. 35: 4878-87.

PMID: # 23361078
Huang, QY. et al. 2013. Connexin43 and angiotensin II alterations in hearts of rats having undergone an acute exposure to alcohol. Am J Forensic Med Pathol. 34: 68-71.

Citations 1 to 10 of 61 total

I was wondering why do we need to discard the DAB solution if precipitates form. I didn't notice the warning before use. I coupled it with a secondary anti-body HRP. However, no band formation was seen for my western blot. Could this be the reason?

Asked by: David Dang
Thank you for your question. We recommend to discard the DAB solution if precipitates are observed prior to use. This is because the DAB should be in solution prior to mixing with the oxidizing agent in order for it to be effective. This is certainly one possibility for why you did not observe any bands after combining with your HRP conjugated secondary antibody, although there are also other possible contributing factors. Please contact our Technical Service Department and we are happy to assist you with troubleshooting your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-16

Hello, I bought thus product and when i tested it didn't work. I diluate dab as recommend in this page but solution continued purple , i teste the pH of tris and it was correct. What did i do wrong?

Asked by: Luã Macedo
Thank you for your question! The solution is fine purple, as long as there is not precipitate. What did you dilute the DAB in? The color should go away/lessen when you add Hydrogen Peroxide. Please contact your local distributor or email us at scbt@scbt.com if you continue to see the purple color with precipitate.
Answered by: Tech Service
Date published: 2017-05-29

What is the composition of this product?

Asked by: hawkeye11
This chemical, DAB, 50X (CAS 7411-49-6), is comprised of DAB, water, and an additive formulation that is non-toxic.
Answered by: Chemical Support 1
Date published: 2017-03-31
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Rated 5 out of 5 by from Various publications cite DAB for its use in HRP Various publications cite DAB for its use in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins. -SCBT Publication Review
Date published: 2015-01-20
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