DAB (3-3′ diaminobenzidine tetrachloride) is suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins. In HRP-based assays, the buffer oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium.
1. Krueger, S.K., et al. 1999. Biotech. Histochem. 74: 105-109. PMID: 10333408
2. Gallyas, F., et al. 1982. J. Histochem. Cytochem. 30: 183-184. PMID: 7061820
3. Larrson, L.I. 1988. Immunocytochemistry: Theory and Practice. Boca Raton, FL. CRC Press, Inc.
See how others have used DAB, 50X. Click on the entry to view the PubMed entry .
PMID: # 26482544
Xing, R. et al. 2016. Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis. Scand. J. Immunol.. 83: 64-71.
PMID: # 27008403
Fan, M. et al. 2016. Rac1/β-Catenin Signalling Pathway Contributes to Trophoblast Cell Invasion by Targeting Snail and MMP9. Cell. Physiol. Biochem.. 38: 1319-32.
PMID: # 27208794
Mukherjee, N. et al. 2016. Frequent inactivation of MCC/CTNNBIP1 and overexpression of phospho-beta-catenin(Y654) are associated with breast carcinoma: Clinical and prognostic significance. Biochim. Biophys. Acta. 1862: 1472-84.
PMID: # 25465073
Maiti, GP. et al. 2015. Frequent inactivation of SLIT2 and ROBO1 signaling in head and neck lesions: clinical and prognostic implications. Oral Surg Oral Med Oral Pathol Oral Radiol. 119: 202-12.
PMID: # 26500110
Zhou, R. et al. 2015. Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars. Experimental cell research. 339: 360-6.
PMID: # 26500110
Zhou, R. et al. 2015. Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars. Exp. Cell Res.. 339: 360-6.
PMID: # 25543042
Wu, J. et al. 2014. The correlation between IL-20 and the Th2 immune response in human asthma. Asian Pac. J. Allergy Immunol.. 32: 316-20.
PMID: # 26339260
Jafari, S. et al. 2014. Recombinant production of mecasermin in E. coli expression system. Research in pharmaceutical sciences. 9: 453-61.
PMID: # 24656731
Zhang, L. et al. 2014. Regeneration of human-ear-shaped cartilage by co-culturing human microtia chondrocytes with BMSCs. Biomaterials. 35: 4878-87.
PMID: # 23361078
Huang, QY. et al. 2013. Connexin43 and angiotensin II alterations in hearts of rats having undergone an acute exposure to alcohol. Am J Forensic Med Pathol. 34: 68-71.
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