Cytokeratin 5/6/18 Antibody (0.N.345) recommended for detection of Cytokeratin 5, Cytokeratin 6 and Cytokeratin 18 of human origin by WB, IP, IF and IHC(P)

Cytokeratin 5/6/18 Antibody (0.N.345): sc-70925

Cytokeratin 5/6/18 Antibody (0.N.345) is rated 4.0 out of 5 by 3.
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Datasheets
    • Cytokeratin 5/6/18 Antibody (0.N.345) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against stratum corneum of human origin
    • recommended for detection of Cytokeratin 5, Cytokeratin 6 and Cytokeratin 18 of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 5/6/18 (0.N.345): sc-70925.
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Can Cytokeratin 5/6/18 (0.N.345): sc-70925 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Cytokeratin 5/6/18 (0.N.345): sc-70925 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good Result I have used in TNBC cell lines for IF and WB. No non-specific band
Date published: 2017-06-08
Rated 4 out of 5 by from WB: Positive data in Hep G2 samples WB: Positive data in Hep G2 samples. -SCBT QC
Date published: 2015-03-23
Rated 4 out of 5 by from Good WB data obtained using HeLa cells Good WB data obtained using HeLa cells. -SCBT QC
Date published: 2015-03-10
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