Cytokeratin 3/2p Antibody (AE5) recommended for detection of Cytokeratin 3 of corneal epithelium and Cytokeratin 2p of palate epithelium of mouse, rat, human, rabbit and bovine origin by WB, IP, IF and IHC(P)

Cytokeratin 3/2p Antibody (AE5): sc-80000

Cytokeratin 3/2p Antibody (AE5) is rated 4.8 out of 5 by 6.
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Datasheets
    • Cytokeratin 3/2p Antibody (AE5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against corneal epithelial Cytokeratin of rabbit origin
    • recommended for detection of Cytokeratin 3 of corneal epithelium and Cytokeratin 2p of palate epithelium of mouse, rat, human, rabbit and bovine origin by WB, IP, IF and IHC(P); useful as a marker to study corneal epithelial differentiation
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 3/2p (AE5): sc-80000.
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I am planning on using this Cytokeratin 3/2p monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from This antibody works This antibody works well for immuno-staining of cultured cell.
Date published: 2019-05-08
Rated 5 out of 5 by from no problem 60 μg mouse brain lystaes loaded,10% SDS PAGE, incubated the antibody 4℃ overnight,ECL System used, expose 1min,can gottarget band,no problem meeting.
Date published: 2017-06-24
Rated 5 out of 5 by from worked great! We observed strong bands in WB at diluted 1:500 sample on bovine eye tissue extract
Date published: 2017-05-20
Rated 5 out of 5 by from Produced expected results Cytokeratin 3/2p Antibody (AE5) produced strong signal by WB and IHC(P)
Date published: 2017-01-17
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human oral mucosa. -SCBT QC
Date published: 2015-01-06
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background bands -SCBT QC
Date published: 2013-08-27
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