cyclin D1 Antibody (DCS-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against recombinant full length human protein
recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact us to receive a FREE 10 µg sample of cyclin D1 (DCS-6): sc-20044.
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The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Is there any possibility to obtain the cytometric protocol that displayed these results?For WB this antibody is very clear, but by Flow cytometry there is no difference in signal fro cyclin D1 negat/pos cells. Any secondary or conjugated recommendation?
Asked by: RobertAlb
Thank you for your inquiry. We recommend working with the conjugated version of the antibody when using it for Flow cytometry. You can refer to our in house protocol on our website here: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Please contact Technical Service by phone, (800)-457-3801 option 2, email <firstname.lastname@example.org>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-11
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
Rated 5 out of
Buena señal.Anticuerpo probado con celulas estrelladas de pancreas. Obtuvimos buena señal.
Date published: 2018-02-14
Rated 5 out of
Quite good antibody!1:200 diluted for wb of rat protein ,the band is quite strong and special!
Date published: 2017-12-30
Rated 4 out of
Works for WBWorks well for western blotting - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of
an excellent antibody for WBThis antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 3 out of
It works but not very wellWe tested this antibody with western blot. The stable expressing cells were tested with control. It works. However, the signal is not strong. Although we successfully detected the target, the sensitivity was not good.
Date published: 2017-09-10
Rated 1 out of
Failed to observe band for regenerating liverI was unable to detect cyclin D1 in pancreatic cell lines Panc1, MiaPaca2, or regenerating liver tissue lysates (36h to 48h) for which I could see PCNA signal increased in a liver regeneration-dependent manner.
Date published: 2017-08-25
Rated 5 out of
NEW LCRC from
WORK to IBIt detects the endogenous Cyclin D1 expression in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. IB(1:100)
Date published: 2017-07-10
Rated 5 out of
Great cell stainingExcellent antibody for use in IF and flow cytometry.
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