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The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
PMID: # 28476031 Cartier, F. et al. 2017. New tumor suppressor microRNAs target glypican-3 in human liver cancer. Oncotarget. 8: 41211-41226.
PMID: # 28166285 Tormos, AM. et al. 2017. p38α regulates actin cytoskeleton and cytokinesis in hepatocytes during development and aging. PLoS ONE. 12: e0171738.
PMID: # 28481876 He, DM. et al. 2017. Oncogenic activity of amplified miniature chromosome maintenance 8 in human malignancies. Oncogene.
PMID: # 28446612 Chen, H. et al. 2017. CDK4 is degraded by APC/C in mitosis and reaccumulates in early G1 phase to initiate a new cell cycle in HeLa cells. J. Biol. Chem..
PMID: # 28486623 Girolimetti, G. et al. 2017. Platinum-induced mitochondrial DNA mutations confer lower sensitivity to paclitaxel by impairing tubulin cytoskeletal organization. Hum. Mol. Genet.. 26: 2961-2974.
PMID: # 28017725 Ishida, S. et al. 2017. Novel mechanism of aberrant ZIP4 expression with zinc supplementation in oral tumorigenesis. Biochem. Biophys. Res. Commun.. 483: 339-345.
PMID: # 27967206 Al-Hendy, A. et al. 2017. Silencing Med12 Gene Reduces Proliferation of Human Leiomyoma Cells Mediated via Wnt/β-Catenin Signaling Pathway. Endocrinology. 158: 592-603.
PMID: # 28634225 Harada, M. et al. 2017. Homeobox Transcription Factor NKX2-1 Promotes Cyclin D1 Transcription in Lung Adenocarcinomas. Mol. Cancer Res..
PMID: # 28498409 Kumarasamy, VM. et al. 2017. Demonstration of a potent RET transcriptional inhibitor for the treatment of medullary thyroid carcinoma based on an ellipticine derivative. Int. J. Oncol.. 51: 145-157.
PMID: # 28790387 Pandey, S. et al. 2017. GSK3β and ERK regulate the expression of 78 kDa SG2NA and ectopic modulation of its level affects phases of cell cycle. Sci Rep. 7: 7555.
Is there any possibility to obtain the cytometric protocol that displayed these results?For WB this antibody is very clear, but by Flow cytometry there is no difference in signal fro cyclin D1 negat/pos cells. Any secondary or conjugated recommendation?
Asked by: RobertAlb
Thank you for your inquiry. We recommend working with the conjugated version of the antibody when using it for Flow cytometry. You can refer to our in house protocol on our website here: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Please contact Technical Service by phone, (800)-457-3801 option 2, email <firstname.lastname@example.org>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-11
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
Rated 5 out of
an excellent antibody for WBThis antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 3 out of
It works but not very wellWe tested this antibody with western blot. The stable expressing cells were tested with control. It works. However, the signal is not strong. Although we successfully detected the target, the sensitivity was not good.
Date published: 2017-09-10
Rated 1 out of
Failed to observe band for regenerating liverI was unable to detect cyclin D1 in pancreatic cell lines Panc1, MiaPaca2, or regenerating liver tissue lysates (36h to 48h) for which I could see PCNA signal increased in a liver regeneration-dependent manner.
Date published: 2017-08-25
Rated 5 out of
NEW LCRC from
WORK to IBIt detects the endogenous Cyclin D1 expression in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. IB(1:100)
Date published: 2017-07-10
Rated 5 out of
Great cell stainingExcellent antibody for use in IF and flow cytometry.
Date published: 2016-12-21
Rated 5 out of
Dewei Ching from
Date published: 2016-12-01
Rated 4 out of
Using for IFUsing for IF - working well.
Date published: 2015-10-08
Rated 5 out of
clean bands seenclean bands seen. great for IF as well!
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