cyclin D1 Antibody (DCS-6) recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

cyclin D1 Antibody (DCS-6): sc-20044

cyclin D1 Antibody (DCS-6) is rated 4.2 out of 5 by 19.
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Datasheets
    • cyclin D1 Antibody (DCS-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against recombinant full length human protein
    • recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of cyclin D1 (DCS-6): sc-20044.
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Is there any possibility to obtain the cytometric protocol that displayed these results?For WB this antibody is very clear, but by Flow cytometry there is no difference in signal fro cyclin D1 negat/pos cells. Any secondary or conjugated recommendation?

Asked by: RobertAlb
Thank you for your inquiry. We recommend working with the conjugated version of the antibody when using it for Flow cytometry. You can refer to our in house protocol on our website here: https://www.scbt.com/scbt/resources/protocols/flow-cytometry Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-22
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Rated 5 out of 5 by from Buena señal. Anticuerpo probado con celulas estrelladas de pancreas. Obtuvimos buena señal.
Date published: 2018-02-14
Rated 5 out of 5 by from Quite good antibody! 1:200 diluted for wb of rat protein ,the band is quite strong and special!
Date published: 2017-12-30
Rated 4 out of 5 by from Works for WB Works well for western blotting - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 3 out of 5 by from It works but not very well We tested this antibody with western blot. The stable expressing cells were tested with control. It works. However, the signal is not strong. Although we successfully detected the target, the sensitivity was not good.
Date published: 2017-09-10
Rated 1 out of 5 by from Failed to observe band for regenerating liver I was unable to detect cyclin D1 in pancreatic cell lines Panc1, MiaPaca2, or regenerating liver tissue lysates (36h to 48h) for which I could see PCNA signal increased in a liver regeneration-dependent manner.
Date published: 2017-08-25
Rated 5 out of 5 by from WORK to IB It detects the endogenous Cyclin D1 expression in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. IB(1:100)
Date published: 2017-07-10
Rated 5 out of 5 by from Great cell staining Excellent antibody for use in IF and flow cytometry.
Date published: 2016-12-21
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