Crystal Violet A component of Gram staining in microorganisms and cell lines

Crystal Violet (CAS 548-62-9)

Crystal Violet | CAS 548-62-9 is rated 5.0 out of 5 by 2.
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Synonym: Gentian Violet; Hexamethylpararosaniline chloride
Application: A component of Gram staining in microorganisms and cell lines
CAS Number: 548-62-9
Molecular Weight: 407.99
Molecular Formula: C25H30N3•Cl
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* Refer to Certificate of Analysis for lot specific data (including water content).
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Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to assess bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines.


References

1. Beveridge, T.J., et al. 1996. Microbiology. 142: 2887-2895. PMID: 8885405
2. Beveridge, T.J. 2001. Biotech Histochem. 76: 111-118. PMID: 11475313
3. Coico, R. 2005. Curr Protoc Microbiol. 3: 3C. PMID: 18770544
4. Bil, J., et al. 2010. Cancer Lett. 288: 57-67. PMID: 19632769

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (50 mg/ml at 27° C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Insoluble in ether.
Storage :
Store at room temperature
Melting Point :
205° C (lit.)(dec.)
Density :
1.19 g/cm3 at 20° C
IC50 :
Sodium Channel, Site 2 radioligand binding: IC50 = 460 nM; Protein Tyrosine Kinase, EGF Receptor enzyme inhibition: IC50 = 473 nM; Muscarinic M5 radioligand binding: IC50 = 535 nM; Alpha-2A adrenergic receptor radioligand binding: IC50 = 536 nM
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
BO9000000
PubChem CID :
11057
Merck Index :
14: 4395
MDL Number :
MFCD00011750
EC Number :
208-953-6
Beilstein Registry :
3580948
SMILES :
[Cl-].CN(C)C1=CC=C(C=C1)C(\\C1=CC=C(C=C1)N(C)C)=C1/C=C\\C(\\C=C/1)=[N+](/C)C

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Certificate of Analysis

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Crystal Violet  Product Citations

See how others have used Crystal Violet. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 26902429  2016. Reproductive sciences (Thousand Oaks, Calif.). 23: 1193-207.

PMID: # 27069628  2016. Pharmacology research & perspectives. 4: e00212.

PMID: # 27087815  2016. Stem cells international. 2016: 5184601.

PMID: # 26238144  2015. Oncology reports. 34: 2022-30.

PMID: # 26314962  Jimbo, M. et al. 2015. Oncotarget. 6: 27312-31.

PMID: # 25955495  2015. Molecular medicine reports. 12: 2451-6.

PMID: # 25872784  Ma, S. et al. 2015. Int. J. Mol. Med. 35: 1561-73.

PMID: # 25371719  Guo, L. et al. 2014. Experimental and therapeutic medicine. 8: 1707-1712.

PMID: # 23376140  Martínez Flores, K. et al. 2013. Toxicol In Vitro. 27: 1031-1042.

PMID: # 23548271  Sahm, F. et al. 2013. Cancer Res. -.

Citations 1 to 10 of 13 total
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Rated 5 out of 5 by from Greater Solublity I am very happy with the product and would like to recommend to friends and colleagues
Date published: 2017-09-05
Rated 5 out of 5 by from Various publications cite the use of Crystal Various publications cite the use of Crystal Violet in Gram staining to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound also helps to asses bacterial contamination of tissue culture samples and can be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines. -SCBT Publication Review
Date published: 2015-01-28
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