CRMP-4 Antibody (LL-5) recommended for detection of CRMP-4 of mouse, rat and human origin by WB, IP and ELISA

CRMP-4 Antibody (LL-5): sc-100323

CRMP-4 Antibody (LL-5) is rated 4.2 out of 5 by 5.
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Datasheets
    • CRMP-4 Antibody (LL-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant CRMP-4 of human origin
    • recommended for detection of CRMP-4 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CRMP-4 (LL-5): sc-100323.
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This antibody has worked well for us for Western Blot. I now want to test CRMP4 levels by immunofluorescence in mouse brain slices. This antibody is only recommended for WB, is there any chance it would work for IF? Or would that be a waste of time?

Asked by: OCC1
Thank you for your question. We have not yet tested this antibody for IF or IHC in tissues or cells, and so this is not a recommended application at this time. However, it certainly may be worth trying since you already have the antibody. The two citations we are aware of currently are using the antibody in Western blot and IP/WB.
Answered by: Technical Support
Date published: 2019-02-25

我想咨询这一款抗体(编号sc-100323)具体识别CRMP4蛋白(共计570个氨基酸)哪一段序列?谢谢!

Asked by: Chutian
感谢您的提问。CRMP-4 Antibody (LL-5): sc-100323免疫原是人源重组CRMP-4,我们没有检测过其具体识别哪一段序列。Thank you for your question. CRMP-4 Antibody (LL-5): sc-100323 is raised against recombinant CRMP-4 of human origin, we do not know which specific sequences this antibody would recognize.
Answered by: Technical Support 8
Date published: 2018-12-21

For Western Blot, is it recommended to use denatured or non-denatured conditions with CRMP-4 (LL-5): sc-100323 antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good specificity Gave a single specific band for endogenous U2OS cell lysate and no band when knocked down using RNAi as expected.
Date published: 2018-03-08
Rated 4 out of 5 by from Strong band in WB in mouse transfected 293T and SK Strong band in WB in mouse transfected 293T and SK-N-MC whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Positive signal in WB in Jurkat whole cell lysate Positive signal in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Good specificity in WB in human transfected Good specificity in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 4 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates and rat brain tissue extract. -SCBT QC
Date published: 2014-02-25
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