CPSF6 Antibody (F-3) recommended for detection of CPSF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CPSF6 Antibody (F-3): sc-376228

CPSF6 Antibody (F-3) is rated 4.9 out of 5 by 7.
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Datasheets
    • CPSF6 Antibody (F-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 167-225 mapping within an internal region of CPSF6 of human origin
    • recommended for detection of CPSF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CPSF6 (F-3): sc-376228.
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I am using CPSF6 (F-3): sc-376228 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked well for WB detected a clear band in WB that went away with siRNA treatment.
Date published: 2019-06-19
Rated 5 out of 5 by from Western blot This antibody works very well. I suggest using 1:1000 dilution to get a nice and clean band.
Date published: 2018-03-15
Rated 5 out of 5 by from an excellent antibody for WB This antibody recognizes a clear and strong band of predicted M.W. on WB (1:500 dilution, 40 ug HeLa extract).
Date published: 2017-09-03
Rated 5 out of 5 by from great antibody for western blot I used this antibody at 1/1000 dilution for western blot in NIH3T3 cells recently, it gives nice and clean band.
Date published: 2017-08-29
Rated 4 out of 5 by from Buena oferta El precio se ajusta a las condiciones de mercado referente a otros anticuerpos.
Date published: 2017-08-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclear Produced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-04-11
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