CPI-17 Antibody (F-4)recommended for detection of CPI-17 of mouse, rat, human and porcine origin by WB, IP, IF and ELISA

CPI-17 Antibody (F-4): sc-48406

CPI-17 Antibody (F-4) is rated 5.0 out of 5 by 2.
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Datasheets
    • CPI-17 Antibody (F-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 88-147 of CPI-17 of human origin
    • recommended for detection of CPI-17 of mouse, rat, human and porcine origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CPI-17 (F-4): sc-48406.
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What is the recommended fixation method for immunofluorescence staining with CPI-17 (F-4): sc-48406 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-29
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