COL1A1 Antibody (C-18) DISCONTINUED: recommended for detection of precursor and mature Collagen α1 Type I of mouse, rat, human and avian origin by WB, IP, IF and ELISA

COL1A1 Antibody (C-18): sc-8784

COL1A1 Antibody (C-18) is rated 4.9 out of 5 by 11.
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    • COL1A1 Antibody (C-18) is a goat polyclonal IgG; 100 µg/ml; or rabbit IgG; 200 µg/ml, sc-8784-R
    • epitope mapping at the C-terminus of Collagen α1 Type I of human origin
    • recommended for detection of precursor and mature Collagen α1 Type I of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
Monos, satisfaction guaranteed

COL1A1 (C-18) has been discontinued and replaced by COL1A1 (3G3): sc-293182. This new monoclonal antibody is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

Ordering Information

COL1A1 (3G3): sc-293182 [ Recommended monoclonal replacement for COL1A1 (C-18) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
COL1A1 Antibody (3G3) sc-293182 100 µg/ml $284.00

at what dilution have this antibody been used?

Asked by: LGrC
Thank you for your question. What dilution we use depends on the application it is being used for, as well as sample type and secondary reagents, but here is a general guideline of starting dilutions (further titration/optimization will be required): Western blot: 1:100 Immunofluorescence/IHC: 1:50 Most other applications, we recommend doing a titration.
Answered by: Tech Service
Date published: 2017-04-12

what is the best blocking agent for this antibody BSA or fat free milk and in which buffer

Asked by: Anonymous
Thank you for your question. Which blocking reagent depends on which application you are using the antibody for. For Western blot we recommend 5% milk with TBS-T. For IHC and IF applications we recommend using blocking serum derived from the same species in which the secondary antibody is raised.
Answered by: Tech Service
Date published: 2018-05-27

My name is Kaho Nomura and I am writing you from Japan. I am a graduate student at Josai University. I have a question about col1a1 antibody(c-18). Please tell me the detailed epitope. Kaho Nomura Josai University

Asked by: kaho
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Rated 5 out of 5 by from sc-8784 quality for research. It works very well in the Western blot analysis. We recommend it to use.
Date published: 2017-07-18
Rated 5 out of 5 by from Perfect function we use this antibody nearly one year, it works very well, and it is trustworthy.
Date published: 2016-11-28
Rated 4 out of 5 by from Quite strong in mouse arteries Quite strong in mouse arteries, Band of interest seen clearly plus some other bands.
Date published: 2015-09-23
Rated 5 out of 5 by from Worked well for WB in mouse Worked well for WB in mouse.
Date published: 2015-08-21
Rated 5 out of 5 by from Worked well with murine tissue Worked well with murine tissue.
Date published: 2015-06-19
Rated 5 out of 5 by from This antibody has been working well for me so far This antibody has been working well for me so far. This is our second time ordering it and we have used it in WB and IF and obtained great results.
Date published: 2015-06-04
Rated 5 out of 5 by from No comment No comment
Date published: 2015-03-23
Rated 5 out of 5 by from Worked well for IF with methanol Worked well for IF with methanol-fixed HS68 cells. -SCBT QC
Date published: 2015-03-04
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