Citric Acid Trisodium Salt Used in the preparation of sodium citrate buffer for antigen unmasking in IHC

Citric Acid Trisodium Salt (CAS 68-04-2)

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Synonym: Trisodium citrate; Sodium citrate
Application: Used in the preparation of sodium citrate buffer for antigen unmasking in IHC
CAS Number: 68-04-2
Purity: ≥99%
Molecular Weight: 258.07
Molecular Formula: C6H5O73Na
* Refer to Certificate of Analysis for lot specific data (including water content).
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As a conjugate base of a weak acid, Citric Acid Trisodium Salt can perform as a biological buffering agent because it resists changes in pH. Citric acid is one of a series of compounds responsible for the physiological oxidation of fats, carbohydrates and proteins to carbon dioxide and water. Often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.
Additional forms available:
Citric Acid Monohydrate (sc-203327)
Citric Acid, Anhydrous (sc-211113)
Sodium Citrate, Dihydrate (sc-203383)
Sodium citrate monobasic (sc-215869)
Sodium citrate tribasic hydrate (sc-236898)
Citrate Concentrated Solution (sc-294091)


References

1. Vandemark, P.J., et al. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243

Usage :
• For antigen retrieval, prepare a solution of 10 mM sodium citrate buffer, pH 6.0.
Appearance :
Powder
Physical State :
Solid
Solubility :
Clear Colorless at 50mg/ml in Water
Storage :
Store at 4° C
Melting Point :
≥150° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
PubChem CID :
6224
MDL Number :
MFCD00012462
EC Number :
200-675-3
SMILES :
C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.[Na+].[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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