Citric Acid, Anhydrous Used in the preparation of citrate buffer for antigen and epitope unmasking in IHC

Citric Acid, Anhydrous  (CAS 77-92-9)

Citric Acid, Anhydrous, CAS 77-92-9 is rated 5.0 out of 5 by 1.
  • y_2019, m_1, d_17, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_211113, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 5ms
  • REVIEWS, PRODUCT
Synonym: Aciletten
Application: Used in the preparation of citrate buffer for antigen and epitope unmasking in IHC
CAS Number: 77-92-9
Purity: >99%
Molecular Weight: 192.13
Molecular Formula: C6H8O7
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Citric Acid is an acidic compound from citrus fruits; as a starting point in the Krebs cycle, citrate is a key intermediate in metabolism. Citric acid is one of a series of compounds responsible for the physiological oxidation of fats, carbohydrates and proteins to carbon dioxide and water. Often used to prepare citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot. May be used to adjust pH and as a sequestering agent for the removal of trace metals.
Additional forms available:
Citric Acid Monohydrate (sc-203327)
Sodium Citrate, Dihydrate (sc-203383)
Citric Acid Trisodium Salt (sc-214745)
Sodium citrate monobasic (sc-215869)
Sodium citrate tribasic hydrate (sc-236898)
Citrate Concentrated Solution (sc-294091)


References

1. Vandemark, P.J., et al. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243

Physical State :
Solid
Solubility :
Soluble in alcohol, water (750 mg/ml at 20° C), and methanol. Insoluble in chloroform, and benzenz.
Storage :
Desiccate at room temperature
Melting Point :
153-159° C (lit.)
Boiling Point :
~309.6° C at 760 mmHg (Predicted)
Density :
1.67 g/cm3 at 20° C
Refractive Index :
n20D 1.58 (Predicted)
IC50 :
Fatso: IC50 = 300 µM (human); SRCAP: IC50 >40 mM (human)
Ki Data :
Beta-lactamase: Ki= 490 µM (Bacillus licheniformis)
pK Values :
pKa: 2.79, pK1: 3.13, pK2: 4.76, pK3: 6.4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
GE7350000
PubChem CID :
311
Merck Index :
14: 2326
MDL Number :
MFCD00011669
EC Number :
201-069-1
Beilstein Registry :
782061
SMILES :
C(C(=O)O)C(CC(=O)O)(C(=O)O)O

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 57ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Various publications cite the use of Citric Acid Various publications cite the use of Citric Acid, Anhydrous to prepare citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. -SCBT Publication Review
Date published: 2015-01-13
  • y_2019, m_1, d_17, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_211113, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 225ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.