Citrate Concentrated Solution 1M; may be used to prepare citrate buffer for antigen unmasking in IHC applications

Citrate Concentrated Solution (CAS 68-04-2)

Citrate Concentrated Solution | CAS 68-04-2 is rated 5.0 out of 5 by 1.
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Application: 1M; may be used to prepare citrate buffer for antigen unmasking in IHC applications
CAS Number: 68-04-2
Molecular Weight: 258.07
Molecular Formula: C6H5Na3O7
* Refer to Certificate of Analysis for lot specific data (including water content).

Citrate Concentrated Solution is 1 M sodium citrate in H2O. As a conjugate base of a weak acid, sodium citrate can perform as a biological buffering agent because it resists changes in pH. May be used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.
Additional forms available:
Citric Acid Monohydrate (sc-203327)
Citric Acid, Anhydrous (sc-211113)
Sodium Citrate, Dihydrate (sc-203383)
Citric Acid Trisodium Salt (sc-214745)
Sodium citrate monobasic (sc-215869)
Sodium citrate tribasic hydrate (sc-236898)


References

1. Vandemark, P.J. and Smith, P.F. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243

Usage :
For antigen retrieval, prepare a solution of 10 mM sodium citrate buffer, pH 6.0.
Formulation :
1 M
Physical State :
Liquid
Storage :
Store at room temperature
Density :
1.14 g/mL
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
PubChem CID :
6224
Merck Index :
14: 8602
MDL Number :
MFCD00012462
EC Number :
200-675-3
SMILES :
C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.[Na+].[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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Citrate Concentrated Solution  Product Citations

See how others have used Citrate Concentrated Solution. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 27468693  Di Siena, S. et al. 2016. Cell Death Dis. 7: e2317.

Citations 1 to 1 of 1 total

Is it already pH adjusted at 6.0?

Asked by: Harsha
Thank you for your question. The pH of Citrate Concentrated Solution (CAS 68-04-2) sc-294091 is not adjusted. The product is supplied as a 1M aquaeous solution. To use it for antigen retrieval we recommend preparing a solution of 10 mM sodium citrate buffer and adjust it to pH 6.
Answered by: Technical Support Europe
Date published: 2019-03-28
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Rated 5 out of 5 by from Various publications cite the use of Citrate Various publications cite the use of Citrate Concentrated Solution in the preparation of citrate buffer for antigen and epitope unmasking in formalin-fixed and paraffin embedded tissue sections. -SCBT Publication Review
Date published: 2015-06-03
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