Cibacron Blue Agarose A reagent used in the isolation of enzymes requiring NAD+ and NADP.

Cibacron Blue Agarose

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Application: A reagent used in the isolation of enzymes requiring NAD<sup>+</sup> and NADP.
Purity: ≥95%
* Refer to Certificate of Analysis for lot specific data (including water content).

Cibacron Blue Agarose is useful in the isolation of enzymes requiring NAD+ and NADP, albumin, interferon, steroid receptors, and 25-Dihydroxyvitamin D3-receptor. It has been shown to bind to several enzymes with known affinities to nucleotide cofactors. Cibacron Blue Agarose has also been shown to bind o dehydrogenases, kinases, restriction endonucleases, albumin, and interferon.


References

1. Kopperschläger, G. et al. 1968. FEBS Lett. 1(3): 137-141. PMID: 11945275
2. Haeckel, R. et al. 1968. Hoppe-Seyler's Z. Physiol. Chem. 349(5): 699-714. PMID: 4386962
3. Thompson, S.T. et al. 1975. Proc. Natl. Acad. Sci. U.S.A. 72(2): 669-672. PMID: 164664
4. Kobayashi, R. and Fang, V.S. 1976. Biochem. Biophys. Res. Commun. 69(4): 1080-1087. PMID: 179540
5. Lamkin, G.E. and King, E.E. 1976. Biochem. Biophys. Res. Commun. 72(2): 560-565. PMID: 186049
6. Travis, J. et al. 1976. Biochem. J. 157(2): 301-306. PMID: 962868
7. Jankowski, W.J. et al. 1976. Biochemistry. 15(23): 5182-5187. PMID: 990274
8. Baksi, K. et al. 1978. Biochemistry. 17(20): 4136-4139. PMID: 708697
9. Robinson, J.B. et al. 1980. Proc. Natl. Acad. Sci. U.S.A. 77(10): 5847-5851. PMID: 6934517
10. Hughes, P. et al. 1982. Biochim. Biophys. Acta. 700(1): 90-100. PMID: 6895701
11. Hanggi, D. and Carr, P. 1985. Anal. Biochem. 149(1): 91-104. PMID: 4073487
12. Scopes, R.K. 1986. J. Chromatogr. 376 : 131-140. PMID: 3711191
13. Burton, S.J., et al., J. Chromatog., v. 435, 127 (1988).
14. Stellwagen, E. 1990. Meth. Enzymol. 182 : 343-357. PMID: 2314247

Appearance :
Suspension
Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
172469
SMILES :
C1=CC=C2C(=C1)C(=O)C3=C(C2=O)C(=C(C=C3NC4=CC(=C(C=C4)NC5=NC(=NC(=N5)NC6=CC=CC=C6S(=O)(=O)O)Cl)S(=O)(=O)O)S(=O)(=O)O)N

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Certificate of Analysis

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Thanks. Note that the Cibacron Blue 3GA used in that paper was purchased from sigma. it was called&nbsp;Cibacron Blue 3GA in particular. Is these two the same product? Thanks.

Asked by: silacgen
Thank you for your question. Yes, the product we offer is the same product referenced in the paper referenced in a previously posted answer.
Answered by: Technical Support
Date published: 2017-05-22

Can it be used for&nbsp;NADH binding ability assay?

Asked by: silacgen
Thank you for your question. Yes, Cibacron Blue Agarose: sc-294028, is suitable for use in a NADH binding ability assay. We have not performed this test ourselves, however there are references in the literature supporting the use of this product for that application. For example, PMID: 21969607, &quot;Tyrosine phosphorylation of lactate dehydrogenase A is important for NADH/NAD(+) redox homeostasis in cancer cells.&quot;
Answered by: Technical Support
Date published: 2017-05-16

What is the concentration of this product?

Asked by: Jz28sail
Contains 2-5 umoles of Cibacron Blue F3GA per ml gel. 4% crosslinked agarose; molecular exclusion limit is approximately 20 million dalton. Specific ligand concentration is determined by aliquot of resin suspended in 75% glycerol and OD260 measured against unsubstituted agarose beads.
Answered by: Chemical Support 5
Date published: 2017-04-04
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