Chromeo P429An easy-to-use amine-reactive pyrylium dye

Chromeo™ P429

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Application: An easy-to-use amine-reactive pyrylium dye
Molecular Weight: 369.82
Molecular Formula: C16H16NOS•ClO4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Chromeo™ P429 detects proteins and peptides by exhibiting a color change. After binding to primary amines, it undergoes a large shortwave spectral shift of 28nm that is visible to the naked eye. There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. The shift of the absorption & emission bands together with the increased fluorescence quantum yield effectively eliminates background from any unbound dye. In addition, because unbound the pyrylium dye is hydrolyzed during the labeling procedure, there is no need for purification following the simple one-step, room-temperature incubation. The labeled protein is ready to use immediately, and any possible background from the unbound dye is eliminated. Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point. Because of these specific features, Chromeo Py-Dyes have been used successfully in a number of "no-wash" applications such as capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing, and as a label in receptor binding studies.


References

1. Craig, D.B. et al. 2005. Electrophoresis. 26(11): 2208-2213. PMID: 15880625
2. Hoffmann, K. et al. 2008. Ann. N. Y. Acad. Sci. 1130 : 28-34. PMID: 18596328
3. Swearingen, K.E. et al. 2008. J Chromatogr A. 1194(2): 249-252. PMID: 18479693
4. Turner, E.H. et al. 2008. J Chromatogr A. 1194(2): 253-256. PMID: 18482729
5. Ramsay, L.M. et al. 2009. Anal. Chem. 81(5): 1741-1746. PMID: 19206532
6. Ramsay, L.M. et al. 2009. Electrophoresis. 30(2): 297-302. PMID: 19204946
7. Dada, O.O. et al. 2010. Anal Bioanal Chem. 397(8): 3305-3310. PMID: 20336452
8. Dickerson, J.A. et al. 2010. Electrophoresis. 31(15): 2650-2654. PMID: 20603830
9. de Jong, S. and Krylov, S.N. 2011. Anal. Chem. 83(16): 6330-6335. PMID: 21728308
10. Keller, M. et al. 2011. Bioorg. Med. Chem. 19(9): 2859-2878. PMID: 21493077
11. de Jong, S. and Krylov, S.N. 2012. Anal. Chem. 84(1): 453-458. PMID: 22107082
12. Johannesen, S.A. et al. 2012. Carbohydr. Res. 352 : 94-100. PMID: 22425443

Physical State :
Solid
Storage :
Store at 4° C
Fluoresence :
λex 457 nm in water; λex 429 nm, λem 536 nm in water (conjugated)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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