CHPT1 Antibody (F-7)recommended for detection of CHPT1 of mouse, rat and human origin by WB, IP, IF and ELISA

CHPT1 Antibody (F-7): sc-515577

CHPT1 Antibody (F-7) is rated 4.0 out of 5 by 2.
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Datasheets
    • CHPT1 Antibody (F-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 17-33 near the N-terminus of CHPT1 of human origin
    • recommended for detection of CHPT1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-515577 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CHPT1 (F-7): sc-515577.
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What application is the blocking peptide sc-515577 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from ヒト細胞サンプルのウェスタンブロッティング 培養ヒト細胞サンプル(Huh7, U2OS)のウェスタンブロッティングに使用しました。明確な反応が得られ、満足しています。
Date published: 2018-07-31
Rated 4 out of 5 by from Review for CHPT1 Antibody Application: Western blot Sample: Liver tissue in Large yellow croaker Gel Running Conditions: 10% SDS-PAGE Loading amount: 20 μg Blocking step: 5% Milk as blocking agent for 2 hour, room temperature Dilution: 1:100 Exposure: 5 minutes Bands: Specific: 45 KDa and 24 KDa
Date published: 2017-06-21
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