CHMP1A Antibody (B-5)recommended for detection of CHMP1A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CHMP1A Antibody (B-5): sc-271617

CHMP1A Antibody (B-5) is rated 5.0 out of 5 by 4.
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Datasheets
    • CHMP1A Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-196 representing full length CHMP1A of human origin
    • recommended for detection of CHMP1A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CHMP1A (B-5): sc-271617.
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I am using CHMP1A (B-5): sc-271617 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from western blot HEK細胞を用いて通常のウエスタンブロットを行い、予想される高さにクリアなシングルバンドを認めました。
Date published: 2018-10-24
Rated 5 out of 5 by from Great specificity This antibody is great! I use it in the Hinck lab for WB and IF. There is no background and always performs well.
Date published: 2018-05-09
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Satisfactory Western Blot data of CHMP1A Satisfactory Western Blot data of CHMP1A expression in non-transfected and mouse CHMP1A transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-10-29
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