Cdc42 Antibody (B-8)recommended for detection of Cdc42 of mouse, rat, human, and d, x origin by WB, IP, IF, IHC(P) and ELISA

Cdc42 Antibody (B-8): sc-8401

Cdc42 Antibody (B-8) is rated 4.5 out of 5 by 33.
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Datasheets
    • Cdc42 Antibody (B-8) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 166-182 at the C-terminus of Cdc42 of human origin
    • recommended for detection of Cdc42 of mouse, rat, human, and d, x origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • blocking peptide, sc-8401 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc42 (B-8): sc-8401.
Submit a review for this product and receive 15 CruzCredits

Could I receive a test sample of your anti Cdc42 (SC-8401) antibody?

Asked by: Timothy
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of this mouse monoclonal antibody, Cdc42 (B-8): sc-8401. Please contact our Technical Service Department to request a sample. Please be prepared to provide your complete shipping address and contact phone number for your lab. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-06-06

I am going to be staining some paraffin embedded sections. What is the recommended secondary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-23
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Rated 3 out of 5 by from Works for western blots Works well for western blots. Did not work for immunoflourescence in our hands, may require standarization
Date published: 2019-03-18
Rated 4 out of 5 by from Good antibody It detects well cdc42 in western blots. It is better to use PVDF membrane for blotting.
Date published: 2019-02-26
Rated 5 out of 5 by from Worked well for immunoblot I used this antibody to stain my membrane for CDC42 in iPSC derived neurons plated for two months.
Date published: 2018-04-24
Rated 4 out of 5 by from specific, but weak The antibody is rather clean with low background in non-milk block. But it is either low titer or low affinity, the signal is very weak even at 1:100.
Date published: 2018-04-13
Rated 5 out of 5 by from Great product! I received this Ab in a couple of months ago and was glad to see it worked nicely! Will definitely purchase when we need it...
Date published: 2018-01-13
Rated 5 out of 5 by from Quite good antibody 1:200 diluted for wb of protein extracted from h9c2 cells ,the band is strong!
Date published: 2017-12-30
Rated 5 out of 5 by from Great Features Good publishable data in WB in chicken cardiac myocytes
Date published: 2017-11-09
Rated 4 out of 5 by from Good to detect Cdc42 I received the Ab a month ago, test the knockdown efficiency of Cdc42. The band is very specific, but a little weak.
Date published: 2017-11-07
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