CD9 Antibody (P1/33/2)recommended for detection of CD9 of mouse, rat and human origin by WB, IP and IF

CD9 Antibody (P1/33/2): sc-20048

CD9 Antibody (P1/33/2) is rated 4.6 out of 5 by 5.
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Datasheets
    • CD9 Antibody (P1/33/2) is a mouse monoclonal mouse IgG1 κ (kappa light chain) provided at 200 µg/ml
    • raised against common acute lymphoblastic leukemia
    • recommended for detection of CD9 of mouse, rat and human origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD9 (C-4): sc-13118 for CD9 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with CD9 (P1/33/2): sc-20048 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good performance Good performance for WB, specific band at 24kda (Hela cell positive control),we suggest not expeosed too long,we have tried different exposed time , too long may caused very high background
Date published: 2017-09-10
Rated 5 out of 5 by from Publishable IHC data obtained in human ABCG2+ Publishable IHC data obtained in human ABCG2+ IGR37 cells xenografts -SCBT Publication Review
Date published: 2015-04-15
Rated 4 out of 5 by from Very large WB band obtained using human PBL Very large WB band obtained using human PBL whole cell lysate. -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed Hela cells. -SCBT QC
Date published: 2015-01-31
Rated 4 out of 5 by from Decent WB signal achieved in HeLa whole cell Decent WB signal achieved in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-29
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