CD8-α Antibody (53-6.7) is a rat monoclonal IgG2a provided at 200 µg/ml
recommended for detection of CD8-α chain of mouse origin by IP, IF, IHC(P) and FCM
available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to either APC, APC-Cy7, PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
available azide-free for in vitro and in vivo depletion, inhibition of T-cell responses to IL-2, and blocking of cytotoxicity (sc-18913 L, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD8-α (53-6.7): sc-18913.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 (CD8-α and CD8-β) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (αα or αβ) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can CD8-alpha Antibody (53-6.7) be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: AbPolly
Yes, CD8-alpha Antibody (53-6.7): sc-18913 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Rated 5 out of
Excellent for FCMGreat, published CD8a antibody. Strong IHC and flow data, and known in vitro and in vivo depletion, inhibition of T-cell responses to IL-2, and blocking of cytotoxicity.
Date published: 2017-01-13
Rated 3 out of
fail to do IHCI bought it for IHC of mouse spleen, thymus and tumor tissues, but failed.
Date published: 2016-10-28
Rated 5 out of
Produced exceptional CD8 scProduced exceptional CD8 sc-18913-APC. -SCBT QC
Date published: 2015-06-01
Rated 4 out of
Publishable data in IHC using mouse skinPublishable data in IHC using mouse skin. -SCBT Publication Review
Date published: 2015-04-29
Rated 4 out of
Publishable data in FCM using mouse t cellsPublishable data in FCM using mouse t cells. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of
Great FCM data of mouse peripheral bloodGreat FCM data of mouse peripheral blood leukocytes. -SCBT QC
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