CD74 Antibody (PIN.1) recommended for detection of the epitope in a region of the cytoplasmic tail of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD74 Antibody (PIN.1): sc-47742

CD74 Antibody (PIN.1) is rated 4.3 out of 5 by 4.
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Datasheets
    • CD74 Antibody (PIN.1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a synthetic peptide DQKPVMDDQRDLISNNE, corresponding to amino acids 12-28 of human CD74
    • recommended for detection of the epitope in a region of the cytoplasmic tail of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD74 (PIN.1): sc-47742 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD74 (PIN.1): sc-47742 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody The CD74 Antibody (PIN.1): sc-47742 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-02-03
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-06-26
Rated 3 out of 5 by from Robust bands seen in WB using BJAB Robust bands seen in WB using BJAB, NAMALWA and IB4 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Produced great immunoperoxidase membrane Produced great immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-02-15
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