CD74 Antibody (M-B741)recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD74 Antibody (M-B741): sc-65272

CD74 Antibody (M-B741) is rated 4.6 out of 5 by 7.
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Datasheets
    • CD74 Antibody (M-B741) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against RPMI 1788 cell line of human origin
    • recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using CD74 (M-B741): sc-65272 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced superb indirect FCM data for BJAB Produced superb indirect FCM data for BJAB cells stained with CD74 . -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Nice band seen in WB using NAMALWA whole Nice band seen in WB using NAMALWA whole cell lysate. -SCBT QC
Date published: 2015-05-29
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-05-25
Rated 5 out of 5 by from Produced excellent immunoperoxidase membrane Produced excellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-05-07
Rated 4 out of 5 by from Strong bands seen in WB using NAMALWA Strong bands seen in WB using NAMALWA, BJAB and IB4 whole cell lysates. -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from Published WB data with human Mel JuSol Published WB data with human Mel JuSol - SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from Produced great indirect Produced great indirect, intracellular FCM data for fixed and permeabilized human peripheral blood leukocytes stained with CD74 . -SCBT QC
Date published: 2013-09-23
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