CD74 Antibody (In-1)recommended for detection of CD74 of mouse origin by WB, IP, IF and FCM

CD74 Antibody (In-1): sc-19627

CD74 Antibody (In-1) is rated 4.3 out of 5 by 8.
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Datasheets
    • CD74 Antibody (In-1) is a rat monoclonal IgG2b provided at 200 µg/ml
    • recommended for detection of CD74 of mouse origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD74 (In-1): sc-19627 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD74 (In-1): sc-19627 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good quality for WB Althrough the antibody dilution suggest from 1:200 to 1:1000,the range is wide.However,the optimal staining conditions can easily be established.finally ,I choose 1:500 dilution for mouse brain tissue detection.
Date published: 2017-07-15
Rated 5 out of 5 by from Clear band seen Clear band seen use this antibody.Also the result is very satisfied us, it give us the right expression level of the controled group and Treatment group(a little blot background).
Date published: 2017-07-08
Rated 5 out of 5 by from Good FCM Clone The FITC conjugated version of this antibody performs well in Flow Cytometry, shows good separation from the normal control.
Date published: 2017-01-27
Rated 4 out of 5 by from Nice results in Flow Nice results in Flow.
Date published: 2015-10-08
Rated 3 out of 5 by from Acceptable FCM data of mouse peripheral blood Acceptable FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-05-24
Rated 3 out of 5 by from Produced nice indirect/Direct FCM data for Produced nice indirect/Direct FCM data for mouse spleen. -SCBT QC
Date published: 2013-07-29
Rated 5 out of 5 by from works great works great
Date published: 2003-06-26
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