CD74 Antibody (D-6)recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CD74 Antibody (D-6): sc-137217

CD74 Antibody (D-6) is rated 4.3 out of 5 by 4.
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Datasheets
    • CD74 Antibody (D-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 17-296 mapping at the C-terminus of CD74 of human origin
    • recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD74 (D-6): sc-137217 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, CD74 (D-6): sc-137217 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from great performance The expression of CD74 in IB4 was analyzed by Western blot analysis with Ramos and Western (B) whole cell lysates and human adrenal tissue extracts (C).
Date published: 2017-01-08
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-05-27
Rated 3 out of 5 by from Robust band seen in WB using BJAB whole cell Robust band seen in WB using BJAB whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-10
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