CD3 Antibody (17A2) recommended for detection of CD3 of mouse origin by IP, IF and FCM

CD3 Antibody (17A2): sc-18843

CD3 Antibody (17A2) is rated 4.0 out of 5 by 4.
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Datasheets
    • CD3 Antibody (17A2) is a rat monoclonal IgG2b provided at 200 µg/ml
    • raised against γδ TCR-positive T-T hybridoma D1 cells of mouse origin
    • recommended for detection of CD3 of mouse origin by IP, IF and FCM
    • available conjugated to FITC for IF, IHC(P) and FCM
    • See CD3 (PC3/188A): sc-20047 for CD3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD3 (17A2): sc-18843 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD3 (17A2): sc-18843 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good in Flow cytometry we used the antibody in Flow cytometry for primary mouse spleen cells, and it's good, but not better than BD's CD3 antibody.
Date published: 2016-12-20
Rated 4 out of 5 by from Wonderful immunofluorescence membrane staining Wonderful immunofluorescence membrane staining in methanol-fixed CTLL-2 cells. -SCBT QC
Date published: 2015-04-30
Rated 4 out of 5 by from Published IHC(pfa) data for use in mouse Published IHC(pfa) data for use in mouse sciatic nerves,nerve roots, spinal cord posterior column samples. -SCBT Publication Review
Date published: 2015-01-21
Rated 4 out of 5 by from Produced good FCM data for mouse peripheral Produced good FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-01-26
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