Cathepsin G Substrate A substrate for quantitative determination of cathepsin G activity

Cathepsin G Substrate (CAS 70967-97-4)

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Synonym: Suc-AAPF-pNA; N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide; N-Succinyl-Ala-Ala-Pro-Phe-pNA
Application: A substrate for quantitative determination of cathepsin G activity
CAS Number: 70967-97-4
Purity: ≥95%
Molecular Weight: 624.7
Molecular Formula: C30H36N6O9
* Refer to Certificate of Analysis for lot specific data (including water content).
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Cathepsin G substrate (Suc-AAPF-pNA) is sensitive colorimetric substrate for chymotrypsin, human pancreatic elastase, human leukocyte cathepsin G, and peptidyl prolyl isomerase. It is also hydrolyzed by cathepsin G and chymase, and used for the quantitative determination of cathepsin G activity.


References

1. Nakajima, K., et al., 1979. Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site. The Journal of biological chemistry. 254(10): 4027-32. PMID: 312290
2. DelMar, E G., et al., 1979. A sensitive new substrate for chymotrypsin. Analytical biochemistry. 99(2): 316-20. PMID: 574722
3. Larcher, G., et al., 1992. Purification and characterization of a fibrinogenolytic serine proteinase from Aspergillus fumigatus culture filtrate. FEBS letters. 308(1): 65-9. PMID: 1644203
4. Bru, R., et al., 1991. Product inhibition of alpha-chymotrypsin in reverse micelles. European journal of biochemistry / FEBS. 199(1): 95-103. PMID: 1712303
5. Kaspar, P., et al., 1984. New photometric assay for chymotrypsin in stool. Clinical chemistry. 30(11): 1753-7. PMID: 6548423
6. Blocher, M., et al., 1999. Modeling of enzymatic reactions in vesicles: the case of alpha-chymotrypsin. Biotechnology and bioengineering. 62(1): 36-43. PMID: 10099511

Usage :
Soluble in N,N-dimethylformamide (DMF) at 25 mg/ml producing a clear, light yellow solution, also soluble in 5% dimethylformamide (v/v) in buffer and in distilled water at 4 mg/ml. A 20 mM solution in dimethyl sulfoxide (DMSO) has been prepared and stored as a stock solution at 4° C. Stock solutions in DMSO were diluted in 0.10 M HEPES buffer, pH 7.5, for the assay of cathepsin G. Soluble at 10 mM in 0.2 M Tris-HCl buffer, pH 8.0. In this buffer, a 1 mM solution of the substrate spontaneously hydrolyzes at a rate of about 0.1% per day at 4° C. It is recommended to prepare aqueous solutions of Cathepsin G substrate immediately prior to use. Aqueous solutions of the substrate should be kept on ice during experiments.
Appearance :
Lyophilized
Physical State :
Solid
Sequence :
Suc-Ala-Ala-Pro-Phe-pNA
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
4381936
SMILES :
CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(CC2=CC=CC=C2)C(=O)NC3=CC=C(C=C3)[N+](=O)[O-])NC(=O)CCC(=O)O

Download SDS (MSDS)

Certificate of Analysis

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