Caspase-3 inhibitor (Z-DEVD-FMK ) is a cell-permeable, potent, and irreversible inhibitor of caspase-3, caspase-6, caspase-7, caspase-8 and caspase-10. Caspase inhibitors play an important role in investigating biological processes. The caspase family of proteins function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Following use of the inhibitor, standard apoptosis assays can then be used to examine treated cells for evidence of apoptosis. For the fluorometric determination of caspase-3 activity, the following caspase apoptosis detection kit is available from Santa Cruz: Caspase-3 Kit (sc-4263 AK).
1. Schlegel, J. et al. 1996. J. Biol. Chem. 271(4): 1841-1844. PMID: 8567626 2. Nicholson, D.W. 1996. Nat. Biotechnol. 14(3): 297-301. PMID: 9630889 3. Masuda, Y. et al. 1997. Biochem. Biophys. Res. Commun. 234(3): 641-645. PMID: 9175767 4. Thornberry, N.A. and Lazebnik, Y. 1998. Science. 281(5381): 1312-1316. PMID: 9721091 5. Brockstedt, E., et al., 1998. The Journal of biological chemistry. 273(43): 28057-64. PMID: 9774422 6. Henshall, D C., et al., 2000. Journal of neurochemistry. 74(3): 1215-23. PMID: 10693954 7. Kugawa, F., et al., 2000. Biological & pharmaceutical bulletin. 23(8): 930-5. PMID: 10963298 8. Nagarajah, N.S. et al. 2004. Mol. Cancer. 3(1): 38. PMID: 15613236 9. Cervantes, J. et al. 2008. Cell. Microbiol. 10(1): 41-52. PMID: 17662073
Pretreatment with an esterase is required when using with purified native or recombinant enzyme.
We recommend a final concentration of 2-10µM for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions.
Soluble in DMSO (13.37 mg/ml).
Store at -20° C
~914.2° C at 760 mmHg (Predicted)
~1.3 g/cm3 (Predicted)
n20D 1.51 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Rated 5 out of
NagarajahNagarajah, NS. et al. (PubMed 15613236) used Caspase-3 Inhibitor (Z-DEVD-fmk) to create a caspase activity profile. Caspase-3 successfully inhibited activity of its target protease caspase-3, but not caspases 8 or 9. -SCBT Publication Review
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