caspase-3 Antibody (E-8) recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA

caspase-3 Antibody (E-8): sc-7272

caspase-3 Antibody (E-8) is rated 4.4 out of 5 by 44.
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Datasheets
    • caspase-3 Antibody (E-8) is a mouse monoclonal IgG2a provided at 200 µg/ml
    • raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin
    • recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact us to receive a FREE 10 µg sample of caspase-3 (E-8): sc-7272.
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I used m-IgGκ BP-HRP: sc-516102 as the secondary antibody, but the bands didn`t existed. The reason is that m-IgGκ BP-HRP: sc-516102 didn`t binded to caspase-3  (E-8): sc-7272, isn`t it ?

Asked by: jmjflying918
Thank you for your question. There are several possibilities that could explain a negative result in Western blot. In this case, one possibility might be that this primary antibody, caspase-3 Antibody (E-8) is a mouse monoclonal with a lambda light chain. If that is the case, then m-IgGκ BP-HRP: sc-516102 would not work. Instead, we would recommend using m-IgGλ BP-HRP: sc-516132 which specifically binds to the lambda light chain of mouse immunoglobulins. We are testing sc-7272 now and will update our product information shortly to specify whether or not this antibody has a kappa or lambda light chain. In the meantime, please contact our Technical Support Department and we would be happy to continue troubleshooting the antibody. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-06

How should I go about double staining a tissue with this antibody if my other primary antibody is also anti-mouse?

Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, caspase-3 Antibody (E-8): sc-7272 is available directly conjugated to HRP (sc-7272 HRP), phycoerythrin (sc-7272 PE), fluorescein (sc-7272 FITC), Alexa Fluor® 488 (sc-7272 AF488), Alexa Fluor® 647 (sc-7272 AF647), TRITC (sc-7272 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-7272 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-28

Which species has this antibody been applied to? Which species does it work for, and which not?

Asked by: DABB
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Rated 1 out of 5 by from Unspecific bands In WB, we got multiple bands, non of which was at the expected size.
Date published: 2018-03-15
Rated 3 out of 5 by from Band intensity in week Antibody worked but the band intensity is week. maybe I should loaded more protein (usually I load 40-50uG).
Date published: 2018-02-01
Rated 5 out of 5 by from Works great for FFPE IHC We got this as a sample. This is an easy, nice working antibody to use. HIER sodium citrate pH 6.0, 1:100 on human heart, and appendix. Looks great!
Date published: 2018-01-04
Rated 5 out of 5 by from This antibody is very good for Western Blot This antibody is very good for Western Blot,and I am so glad to use it!
Date published: 2017-12-06
Rated 1 out of 5 by from Fluorescent IHC did not worked Fluorescent IHC on mouse retina frozen sections (10 um) did not work Used at 1/100
Date published: 2017-11-06
Rated 4 out of 5 by from Very Good Antibody I used this to determine the Caspase-3 levels expressed in melanoma cell lines MeWo and SK-MEL-2. WB worked well with 1:500 dilution, (4% BSA, 0.05% TBST).
Date published: 2017-10-24
Rated 4 out of 5 by from good product The bands with this antibody are stronger than the other two caspase-3 antibodies ( sc-271759 and sc-373730 ) in my system. The primary antibody were diluted at 1:2000 and incubated with NC membrane at room temperature. The ph value is 7.45.
Date published: 2017-09-30
Rated 3 out of 5 by from 还好吧 申请了该抗体的单克隆试用装。用的大鼠的组织标本做的WB,条带的特异性好,首次使用,背景不高,但不能检测cleaved caspase 3,有点小缺陷。
Date published: 2017-09-25
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