caspase-3 Antibody (E-8) is a mouse monoclonal IgG2a provided at 200 µg/ml
raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin
recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
Contact us to receive a FREE 10 µg sample of caspase-3 (E-8): sc-7272.
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Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Which species has this antibody been applied to? Which species does it work for, and which not?
Asked by: DABB
Thanks for your question. The caspase-3 (E-8) antibody is recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA. Please contact Technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
I used m-IgGκ BP-HRP: sc-516102 as the secondary antibody, but the bands didn`t existed. The reason is that m-IgGκ BP-HRP: sc-516102 didn`t binded to caspase-3 (E-8): sc-7272, isn`t it ?
Asked by: jmjflying918
Thank you for your question. There are several possibilities that could explain a negative result in Western blot. In this case, one possibility might be that this primary antibody, caspase-3 Antibody (E-8) is a mouse monoclonal with a lambda light chain. If that is the case, then m-IgGκ BP-HRP: sc-516102 would not work. Instead, we would recommend using m-IgGλ BP-HRP: sc-516132 which specifically binds to the lambda light chain of mouse immunoglobulins. We are testing sc-7272 now and will update our product information shortly to specify whether or not this antibody has a kappa or lambda light chain. In the meantime, please contact our Technical Support Department and we would be happy to continue troubleshooting the antibody. Please call our Asian Support office toll-free at 800-820-8626, email firstname.lastname@example.org, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-06
How should I go about double staining a tissue with this antibody if my other primary antibody is also anti-mouse?
Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, caspase-3 Antibody (E-8): sc-7272 is available directly conjugated to HRP (sc-7272 HRP), phycoerythrin (sc-7272 PE), fluorescein (sc-7272 FITC), Alexa Fluor® 488 (sc-7272 AF488), Alexa Fluor® 647 (sc-7272 AF647), TRITC (sc-7272 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-7272 AF405, 100 µg/2 ml).
Rated 1 out of
Unspecific bandsIn WB, we got multiple bands, non of which was at the expected size.
Date published: 2018-03-15
Rated 3 out of
Band intensity in weekAntibody worked but the band intensity is week. maybe I should loaded more protein (usually I load 40-50uG).
Date published: 2018-02-01
Rated 5 out of
Works great for FFPE IHCWe got this as a sample. This is an easy, nice working antibody to use. HIER sodium citrate pH 6.0, 1:100 on human heart, and appendix. Looks great!
Date published: 2018-01-04
Rated 5 out of
This antibody is very good for Western BlotThis antibody is very good for Western Blot,and I am so glad to use it!
Date published: 2017-12-06
Rated 1 out of
Fluorescent IHC did not workedFluorescent IHC on mouse retina frozen sections (10 um) did not work
Used at 1/100
Date published: 2017-11-06
Rated 4 out of
Very Good AntibodyI used this to determine the Caspase-3 levels expressed in melanoma cell lines MeWo and SK-MEL-2. WB worked well with 1:500 dilution, (4% BSA, 0.05% TBST).
Date published: 2017-10-24
Rated 4 out of
good productThe bands with this antibody are stronger than the other two caspase-3 antibodies ( sc-271759 and sc-373730 ) in my system. The primary antibody were diluted at 1:2000 and incubated with NC membrane at room temperature. The ph value is 7.45.
Date published: 2017-09-30
Rated 3 out of
还好吧申请了该抗体的单克隆试用装。用的大鼠的组织标本做的WB，条带的特异性好，首次使用，背景不高，但不能检测cleaved caspase 3，有点小缺陷。
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