Caspase-1 inhibitor VI (Z-YVAD-FMK) is a cell-permeable, irreversible inhibitor of caspase-1, caspase-4, and caspase-5 activity. Caspase inhibitors play an important role in investigating biological processes. The caspase family of proteins function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. Following use of the inhibitor, standard apoptosis assays can be used to examine treated cells for evidence of apoptosis. Additional caspase-1 inhibitors available: Caspase-1 inhibitor (sc-3069) Caspase-1 Inhibitor II (sc-300323) Caspase-1 Inhibitor IV (sc-300324) Caspase-1 Inhibitor I (sc-358878)
1. Auerbuch, V. et al. 2009. PLoS Pathog. 5(12): e1000686. PMID: 19997504 2. Brenner, B., et al. 1998. Cell Death Differ. 5: 29-37. PMID: 10200443 3. Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273: 32608-32613. PMID: 9829999 4. Hilbi, H., et al. 1998. J. Biol. Chem. 273: 32895-32900. PMID: 9830039 5. Thornberry, N.A, et al. 1998. Science. 281: 1312-1316. PMID: 9721091 6. Schlegel, J., et al. 1996. J. Biol. Chem. 271: 1841-1844. PMID: 8567626 7. Enari, M., et al. 1995. Nature. 375: 78-81. PMID: 7536900 8. Walker, N.P., et al. 1994. Cell. 78: 343-352. PMID: 8044845 9. Thornberry, N.A., et al. 1992. Nature. 356: 768-774. PMID: 1574116 10. Pirnia, F. et al. 2006. Anticancer Res. 26(3A): 1765-1772. PMID: 16827105
The carboxyl groups are OMe ester forms to enhance cell permeability. If purified enzyme is used, esterase should be added for the hydrolysis of the ester groups.
Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO.
Store solution aliquots at -20°C.
Soluble in DMSO.
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Can Caspase-1 inhibitor VI be used in animal tissues?
Asked by: ChemSynth123
Thank you for your question.
Caspase-1 inhibitor VI, sc-3071, can be used in animal tissue. Please refer to the following reference: 10. Pirnia, F. et al. 2006. Anticancer Res. 26(3A): 1765-1772. PMID: 16827105. SFJ
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