Caspase-1 Inhibitor II An inhibitor of caspase-1, caspase-4, and caspase-5

Caspase-1 Inhibitor II (CAS 178603-78-6)

Caspase-1 Inhibitor II | CAS 178603-78-6 is rated 5.0 out of 5 by 1.
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Synonym: Ac-YVAD-CMK
Application: An inhibitor of caspase-1, caspase-4, and caspase-5
CAS Number: 178603-78-6
Molecular Weight: 540.99
Molecular Formula: C24H33ClN4O8
* Refer to Certificate of Analysis for lot specific data (including water content).
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Caspase-1 Inhibitor II (Ac-YVAD-CMK) is a cell-permeable and irreversible inhibitor of caspase-1 (Ki = 760 pM), caspase-4, and caspase-5. It also inhibits Fas-mediated apoptosis and acidic sphingomyelinase activation. Caspase inhibitors play an important role in investigating biological processes. The caspase family of proteins function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. Following use of the inhibitor, standard apoptosis assays can be used to examine treated cells for evidence of apoptosis.
Additional caspase-1 inhibitors available:
caspase-1 inhibitor (sc-3069)
caspase-1 inhibitor VI (sc-3071)
Caspase-1 Inhibitor IV (sc-300324)
Caspase-1 Inhibitor I (sc-358878)


References

1. Brenner, B., et al. 1998. Cell Death Differ. 5: 29-37. PMID: 10200443
2. Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273: 32608-32613. PMID: 9829999
3. Hilbi, H., et al. 1998. J. Biol. Chem. 273: 32895-32900. PMID: 9830039
4. Thornberry, N.A., et al. 1998. Science. 281: 1312-1316. PMID: 9721091
5. Schlegel, J., et al. 1996. J. Biol. Chem. 271: 1841-1844. PMID: 8567626
6. Enari, M., et al. 1995. Nature. 375: 78-81. PMID: 7536900
7. Walker, N.P., et al. 1994. Cell. 78: 343-352. PMID: 8044845
8. Thornberry, N.A., et al. 1992. Nature. 356: 768-774. PMID: 1574116
9. Wu, Bihua., et al., 2010. Ac-YVAD-CMK Decreases Blood-Brain Barrier Degradation by Inhibiting Caspase-1 Activation of Interleukin-1β in Intracerebral Hemorrhage Mouse Model. Translational stroke research. 1(1): 57-64. PMID: 20596246

Physical State :
Solid
Sequence :
Ac-Tyr-Val-Ala-Asp-CMK
Solubility :
Soluble in DMSO (~20 mg/ml), water (0.25 mg/ml) at 25 °C, PBS pH7.2 (~0.25 mg/ml), and DMF (~10 mg/ml).
Storage :
Store at -20° C
Boiling Point :
969.07° C at 760 mmHg (Predicted)
Density :
1.31 g/cm3 (Predicted)
Refractive Index :
n20D 1.55 (Predicted)
Ki Data :
caspase-1: Ki= 760 pM
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
AY3079566
PubChem CID :
9915279
MDL Number :
MFCD00237124
SMILES :
C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)CCl)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)C

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Certificate of Analysis

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What is this soluble in? How long can it be stored?

Asked by: chemsd02
Thank you for your question. sc-300323 (Caspase-1 Inhibitor II, CAS 178603-78-6) is soluble in DMSO (20 mg/mL) and DMF (10 mg/mL). After reconstituting, aliquot and freeze at -20ºC. Stock solutions are stable for up to 3 months when stored at -20ºC.
Answered by: Chemical Support 10
Date published: 2017-04-06
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Rated 5 out of 5 by from Wu Wu, B. et al. (PubMed 20596246) tested the neuroprotective effects of Ac-YVAD-CMK (Caspase-1 Inhibitor II), in a mouse model of intracerebral hemorrhage (ICH). Ac-YVAD-CMK treatment significantly reduced brain edema and improved neurological functions. The neuroprotection was associated with downregulation of IL-1 , JNK, MMP-9, and an inhibition of ZO-1 degradation in brain. -SCBT Publication Review
Date published: 2015-05-07
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