Caspase-1 Inhibitor I (Ac-YVAD-CHO, also designated L-709049) is a potent, specific, reversible inhibitor of caspase-1 (Ki = 200 pM for human recombinant caspase-1), caspase-4, and caspase-5. Caspase inhibitors play an important role in investigating biological processes. The caspase family of proteins function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. Ac-YVAD-CHO has been shown to strongly inhibit anti-APO-1 induced apoptosis in L929-APO-1 cells. Following use of the inhibitor, standard apoptosis assays can be used to examine treated cells for evidence of apoptosis.
Additional caspase-1 inhibitors available:
caspase-1 inhibitor (sc-3069)
caspase-1 inhibitor VI (sc-3071)
Caspase-1 Inhibitor II (sc-300323)
Caspase-1 Inhibitor IV (sc-300324)
1. Brenner, B., et al. 1998. Cell Death Differ. 5: 29-37. PMID: 10200443
2. Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273: 32608-32613. PMID: 9829999
3. Hilbi, H., et al. 1998. J. Biol. Chem. 273: 32895-32900. PMID: 9830039
4. Thornberry, N.A, et al. 1998. Science. 281: 1312-1316. PMID: 9721091
5. Schlegel, J., et al. 1996. J. Biol. Chem. 271: 1841-1844. PMID: 8567626
6. Enari, M., et al. 1995. Nature. 375: 78-81. PMID: 7536900
7. Walker, N.P., et al. 1994. Cell. 78: 343-352. PMID: 8044845
8. Thornberry, N.A., et al. 1992. Nature. 356: 768-774. PMID: 1574116
Aqueous solutions should not be stored more than 1 day.
Soluble in DMSO (5 mg/ml), water (5 mg/ml) at 25 °C, ethanol (~30 mg/ml), DMF (~30 mg/ml), and PBS(pH 7.2) (~5 mg/ml).
How should be this product（Caspase-1 inhibitor I sc-358878） used in mice
Asked by: xiehaibo666
All of our product can not be used in vivo experiment.
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Rated 5 out of
The Caspase-1 inhibitor works wellWe bought this inhibitor and used it to pretreat the THP1 cells for 1 h with 20 um, and then treated with LPS/ATP. The inhibitor blocked the IL-1b production induced by LPS/ATP.
Date published: 2018-07-31
Rated 5 out of
Wang et alWang et al. (PubMed ID 26071315) used Caspase-1 inhibitor I to decrease the expression of CD80, CD86 and MHC class II on dendritic cells and to ameliorate autoimmune myasthenia gravis. -SCBT Publication Review
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