Caspase-1 inhibitor I An inhibitor of caspase-1, caspase-4, and caspase-5

Caspase-1 inhibitor I (CAS 143313-51-3)

Caspase-1 inhibitor I | CAS 143313-51-3 is rated 5.0 out of 5 by 2.
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Synonym: N-Ac-Tyr-Val-Ala-Asp-CHO; Ac-YVAD-CHO
Application: An inhibitor of caspase-1, caspase-4, and caspase-5
CAS Number: 143313-51-3
Purity: ≥95%
Molecular Weight: 492.52
Molecular Formula: C23H32N4O8
* Refer to Certificate of Analysis for lot specific data (including water content).
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Caspase-1 Inhibitor I (Ac-YVAD-CHO, also designated L-709049) is a potent, specific, reversible inhibitor of caspase-1 (Ki = 200 pM for human recombinant caspase-1), caspase-4, and caspase-5. Caspase inhibitors play an important role in investigating biological processes. The caspase family of proteins function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. Ac-YVAD-CHO has been shown to strongly inhibit anti-APO-1 induced apoptosis in L929-APO-1 cells. Following use of the inhibitor, standard apoptosis assays can be used to examine treated cells for evidence of apoptosis.

Additional caspase-1 inhibitors available:
caspase-1 inhibitor (sc-3069)
caspase-1 inhibitor VI (sc-3071)
Caspase-1 Inhibitor II (sc-300323)
Caspase-1 Inhibitor IV (sc-300324)


References

1. Brenner, B., et al. 1998. Cell Death Differ. 5: 29-37. PMID: 10200443
2. Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273: 32608-32613. PMID: 9829999
3. Hilbi, H., et al. 1998. J. Biol. Chem. 273: 32895-32900. PMID: 9830039
4. Thornberry, N.A, et al. 1998. Science. 281: 1312-1316. PMID: 9721091
5. Schlegel, J., et al. 1996. J. Biol. Chem. 271: 1841-1844. PMID: 8567626
6. Enari, M., et al. 1995. Nature. 375: 78-81. PMID: 7536900
7. Walker, N.P., et al. 1994. Cell. 78: 343-352. PMID: 8044845
8. Thornberry, N.A., et al. 1992. Nature. 356: 768-774. PMID: 1574116

Usage :
Aqueous solutions should not be stored more than 1 day.
Physical State :
Solid
Solubility :
Soluble in DMSO (5 mg/ml), water (5 mg/ml) at 25 °C, ethanol (~30 mg/ml), DMF (~30 mg/ml), and PBS(pH 7.2) (~5 mg/ml).
Storage :
Store at -20° C
Melting Point :
349.84 °C (Predicted)
Boiling Point :
~937.75 °C at 760 mmHg (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.55 (Predicted)
IC50 :
Caspase-1: IC50 = 8.4 nM (human); PBMC : IC50 = 1.2 µM (human); Interleukin-1 beta: IC50 = 2 µM (human)
Ki Data :
recombinant caspase-1: Ki= 200 pM (human); Caspase-1: Ki= 9 nM (mouse)
pK Values :
pKb: 4.20 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
5311139
MDL Number :
MFCD00237123
SMILES :
C[C@@H](C(=O)N[C@@H](CC(=O)O)C=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)C

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Certificate of Analysis

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Caspase-1 inhibitor I  Product Citations

See how others have used Caspase-1 inhibitor I. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 27628063  Winges, A. et al. 2016. Graefes Arch. Clin. Exp. Ophthalmol. 254: 2387-2400.

PMID: # 26086368  Liang, Y. et al. 2015. Brain Res. 1622: 43-50.

PMID: # 24340123  Tavares, AH. et al. 2013. PLoS Negl Trop Dis. 7: e2595.

PMID: # 17235324  Park, HJ. et al. 2007. J Invest Dermatol. 127(5): 1210-1216.

Citations 1 to 4 of 4 total

How should be this product(Caspase-1 inhibitor I sc-358878) used in mice

Asked by: xiehaibo666
All of our product can not be used in vivo experiment. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 10
Date published: 2017-11-28
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Rated 5 out of 5 by from The Caspase-1 inhibitor works well We bought this inhibitor and used it to pretreat the THP1 cells for 1 h with 20 um, and then treated with LPS/ATP. The inhibitor blocked the IL-1b production induced by LPS/ATP.
Date published: 2018-07-31
Rated 5 out of 5 by from Wang et al Wang et al. (PubMed ID 26071315) used Caspase-1 inhibitor I to decrease the expression of CD80, CD86 and MHC class II on dendritic cells and to ameliorate autoimmune myasthenia gravis. -SCBT Publication Review
Date published: 2015-06-10
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