Date published: 2025-9-15
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Cas9 Antibody (7A9-3A3) - Western Blotting - Image 384064
Cas9 Antibody (7A9-3A3): sc-517386. Western blot analysis of Cas9 expression in non-transfected: sc-117752 (A) and Streptococcus pyogenes Cas9-transfected (B, C & D) 293T whole cell lysates. CRISPR/Cas9 plasmids used: sc-400099 (B), sc-400193 (C) and sc-413603 (D).
Cas9 Antibody (7A9-3A3) - Western Blotting - Image 384064
Cas9 Antibody (7A9-3A3): sc-517386. Western blot analysis of Cas9 expression in non-transfected: sc-117752 (A) and Streptococcus pyogenes Cas9-transfected (B, C & D) 293T whole cell lysates. CRISPR/Cas9 plasmids used: sc-400099 (B), sc-400193 (C) and sc-413603 (D).
Cas9 Antibody (7A9-3A3) - Western Blotting - Image 395718
Cas9 Antibody (7A9-3A3) HRP: sc-517386 HRP. Direct western blot analysis of Cas9 expression in non-transfected: sc-117752 (A) and Streptococcus pyogenes Cas9-transfected (B and C) 293T whole sell lysates. CRISPR/Cas9 plasmids used: sc-400193 (B) and sc-413603 (C).
Cas9 Antibody (7A9-3A3): sc-517386. Western blot analysis of Cas9 expression in non-transfected: sc-117752 (A) and Streptococcus pyogenes Cas9-transfected (B, C & D) 293T whole cell lysates. CRISPR/Cas9 plasmids used: sc-400099 (B), sc-400193 (C) and sc-413603 (D).

Cas9 Antibody (7A9-3A3): sc-517386

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Datasheets
  • Cas9 Antibody (7A9-3A3) is a mouse monoclonal IgG1 κ Cas9 antibody, cited in 31 publications, provided at 200 µg/ml
  • raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin
  • Cas9 Antibody (7A9-3A3) is recommended for detection of transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) of Streptococcus pyogenes origin by WB, IP, IF, IHC(P) and FCM
  • Anti-Cas9 Antibody (7A9-3A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cas9 (7A9-3A3): sc-517386.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cas9 Antibody (7A9-3A3) for WB and IHC(P) applications. These reagents are now offered in bundles with Cas9 Antibody (7A9-3A3) (see ordering information below).

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Research Information

SEE ALSO...

Cas9 Antibody (7A9-3A3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Cas9 protein of Streptococcus pyogenes origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Cas9 antibody (7A9-3A3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cas9, a 1,368 amino acid RNA-guided endonuclease, plays a crucial role in the CRISPR-Cas9 gene-editing system, enabling precise modifications to DNA sequences. Cas9′s ability to create double-strand breaks at specific genomic locations is vital for targeted gene disruption, allowing researchers to study gene function and develop potential therapeutic strategies. Cas9′s structure includes a recognition lobe and a nuclease lobe, which are essential for interaction with guide RNA and target DNA, ensuring high specificity and efficiency in gene editing applications. Anti-Cas9 antibody (7A9-3A3) serves as a powerful tool in molecular biology, biotechnology, and therapeutic development, paving the way for advancements in genetic research and treatment of genetic disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Cas9 Antibody (7A9-3A3) References:

  1. CRISPR/Cas system and its role in phage-bacteria interactions.  |  Deveau, H., et al. 2010. Annu Rev Microbiol. 64: 475-93. PMID: 20528693
  2. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III.  |  Deltcheva, E., et al. 2011. Nature. 471: 602-7. PMID: 21455174
  3. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.  |  Jinek, M., et al. 2012. Science. 337: 816-21. PMID: 22745249
  4. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria.  |  Gasiunas, G., et al. 2012. Proc Natl Acad Sci U S A. 109: E2579-86. PMID: 22949671
  5. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.  |  Sternberg, SH., et al. 2014. Nature. 507: 62-7. PMID: 24476820
  6. Crystal structure of Cas9 in complex with guide RNA and target DNA.  |  Nishimasu, H., et al. 2014. Cell. 156: 935-49. PMID: 24529477
  7. Development and applications of CRISPR-Cas9 for genome engineering.  |  Hsu, PD., et al. 2014. Cell. 157: 1262-1278. PMID: 24906146
  8. Unravelling the structural and mechanistic basis of CRISPR-Cas systems.  |  van der Oost, J., et al. 2014. Nat Rev Microbiol. 12: 479-92. PMID: 24909109
  9. [Changes in lymphocyte subpopulations in relation to anesthesia procedure].  |  Käbisch, S., et al. 1986. Anasth Intensivther Notfallmed. 21: 327-32. PMID: 2949671

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cas9 Antibody (7A9-3A3)

sc-517386
200 µg/ml
$316.00

Cas9 Antibody (7A9-3A3): m-IgG Fc BP-HRP Bundle

sc-531580
200 µg Ab; 10 µg BP
$354.00

Cas9 Antibody (7A9-3A3): m-IgGκ BP-HRP Bundle

sc-525309
200 µg Ab, 40 µg BP
$354.00

Cas9 Antibody (7A9-3A3): m-IgG1 BP-HRP Bundle

sc-544637
200 µg Ab; 20 µg BP
$354.00

Cas9 Antibody (7A9-3A3) AC

sc-517386 AC
500 µg/ml, 25% agarose
$416.00

Cas9 Antibody (7A9-3A3) HRP

sc-517386 HRP
200 µg/ml
$316.00

Cas9 Antibody (7A9-3A3) FITC

sc-517386 FITC
200 µg/ml
$330.00

Cas9 Antibody (7A9-3A3) PE

sc-517386 PE
200 µg/ml
$343.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 488

sc-517386 AF488
200 µg/ml
$357.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 546

sc-517386 AF546
200 µg/ml
$357.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 594

sc-517386 AF594
200 µg/ml
$357.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 647

sc-517386 AF647
200 µg/ml
$357.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 680

sc-517386 AF680
200 µg/ml
$357.00

Cas9 Antibody (7A9-3A3) Alexa Fluor® 790

sc-517386 AF790
200 µg/ml
$357.00

Could I use this for flow?

Asked by: NilaM
Thank you for your question. Yes, the antibody is recommended for WB, IP, IF, IHC(P) and FCM.
Answered by: BlakeJ
Date published: 2023-08-02

How I could have a free sample for immunofluorescent staining?

Asked by: VcPb
Thank you for your question. To arrange for a free sample of this antibody, please contact our technical service team directly. Please note that only unconjugated antibodies can be supplied as samples.
Answered by: Tech Support Europe
Date published: 2022-12-01

Do you provide sample for us to try? Thank you!

Asked by: Longjun
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-12-20

As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.

Asked by: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03

Is there any validation of this antibody working for immunofluorescence applications?

Asked by: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Answered by: Technical Support
Date published: 2019-03-13

What part of the N-terminus was this antibody raised against?

Asked by: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Answered by: Technical Support
Date published: 2020-12-20
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Rated 5 out of 5 by from Works for Western blotThe antibody is effective for SDS immunoblotting. To dilute the primary antibody, we used a ratio of 1:500 in a mixture of 5% nonfat dry milk, 1X TBS, and 0.1% Tween® 20. The antibody was left overnight at 4°C.
Date published: 2023-11-07
Rated 5 out of 5 by from Good antibody for WBI used 1:1000. It works good with my cells transfected with dCAS9 plasmid.
Date published: 2022-01-25
Rated 5 out of 5 by from This antibody sample worked wellThis cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459. 1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date published: 2018-09-25
Rated 5 out of 5 by from Great antibodyI got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date published: 2018-02-22
Rated 4 out of 5 by from 还不错我一个月前申请可改抗体的试用装，很高兴可以提供试用反馈，这个抗体总体不错，有杂带，但是主带清晰，不受干扰。
Date published: 2017-09-27
Rated 5 out of 5 by from successful resultI have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃，if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
Date published: 2017-07-23
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Cas9 Antibody (7A9-3A3) is rated 4.8 out of 5 by 6.
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