CAPSO (N-cyclohexyl-2-hydroxyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPSO buffer, a zwitterionic buffer that is useful in the range of pH 8.9 - 10.3. CAPSO buffer is used in western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPSO is not one of the original Good's buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.
CAPSO sodium salt(sc-285280)
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See how others have used CAPSO. Click on the entry to view the PubMed entry .
PMID: # 16687400 Radchenko, MV. et al. 2006. J. Biol. Chem. 281: 19822-19829.
PMID: # 12547799 Váró, G. et al. 2003. Biophys. J. 84: 1202-1207.
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