c-Kit Antibody (Ab 81) recommended for detection of c-Kit of human origin by WB, IP, IF, FCM and ELISA

c-Kit Antibody (Ab 81): sc-13508

c-Kit Antibody (Ab 81) is rated 5.0 out of 5 by 7.
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Datasheets
    • c-Kit Antibody (Ab 81) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length c-Kit of human origin
    • recommended for detection of c-Kit of human origin by WB, IP, IF, FCM and ELISA
    • available conjugated to agarose for IP; to HRP for WB, IHC(P) and ELISA; and to phycoerythrin for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of c-Kit (Ab 81): sc-13508.
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What negative control should be used when using the conjugated version of this antibody in flow cytometry?

Asked by: cjMara
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-13508 PE, then you would want to use normal mouse IgG1 PE, sc-2866. https://www.scbt.com/scbt/product/normal-mouse-igg1-pe
Answered by: Technical Support 7
Date published: 2017-01-20
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Rated 5 out of 5 by from Superb Great product for western blot;IF, FCM and IHC staining applications
Date published: 2017-02-05
Rated 5 out of 5 by from Very strong antibody! Versatile and reliable, this antibody is a great choice for all your c-Kit detection needs. Great for WB, IHC and Flow Cytometry!
Date published: 2017-01-09
Rated 5 out of 5 by from Good staining in IHC in human GIST cells Good staining in IHC in human GIST cells. -SCBT Publication Review
Date published: 2015-03-16
Rated 5 out of 5 by from Positive signal in WB in HEL 92 Positive signal in WB in HEL 92.1.17 and TT whole cell lysate. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Strong band in WB in CCRF Strong band in WB in CCRF-HSB-2 and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane staining in methanol-fixed TF-1 cells. -SCBT QC
Date published: 2015-01-11
Rated 5 out of 5 by from Produced nice FCM data for TF Produced nice FCM data for TF-1 cells. -SCBT QC
Date published: 2013-11-24
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