c-Kit Antibody (2B8) recommended for detection of c-Kit of mouse and rat origin by IF and FCM

c-Kit Antibody (2B8): sc-19619

c-Kit Antibody (2B8) is rated 5.0 out of 5 by 4.
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Datasheets
    • c-Kit Antibody (2B8) is a rat monoclonal rat IgG2b provided at 200 µg/ml
    • raised against bone marrow mast cells of mouse origin
    • recommended for detection of c-Kit of mouse and rat origin by IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See c-Kit (E-3): sc-365504 for c-Kit antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can c-Kit (2B8): sc-19619 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, c-Kit (2B8): sc-19619 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-01-29
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Rated 5 out of 5 by from Good publishable data in IHC in mouse liver Good publishable data in IHC in mouse liver. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Worked well in flow in rat hepatocytes Worked well in flow in rat hepatocytes. -SCBT Publication Review
Date published: 2015-03-06
Rated 5 out of 5 by from Good staining in IF in C57BL/6J (B6) mice Good staining in IF in C57BL/6J (B6) mice in Marrow-derived cells. -SCBT Publication Review
Date published: 2015-01-30
Rated 5 out of 5 by from Excellent FCM analysis of mouse bone marrow cells Excellent FCM analysis of mouse bone marrow cells. -SCBT QC
Date published: 2013-04-11
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